Blood outgrowth endothelial cells alter remodeling of completely biological engineered grafts implanted into the sheep femoral artery

Lee A. Meier, Zeeshan H. Syedain, Matthew T. Lahti, Sandra S. Johnson, Minna H. Chen, Robert P. Hebbel, Robert T. Tranquillo

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Hemocompatibility of tissue-engineered vascular grafts remains a major hurdle to clinical utility for small-diameter grafts. Here we assessed the feasibility of using autologous blood outgrowth endothelial cells to create an endothelium via lumenal seeding on completely biological, decellularized engineered allografts prior to implantation in the sheep femoral artery. The 4-mm-diameter, 2- to 3-cm-long grafts were fabricated from fibrin gel remodeled into an aligned tissue tube in vitro by ovine dermal fibroblasts prior to decellularization. Decellularized grafts pre-seeded with blood outgrowth endothelial cells (n=3) retained unprecedented (>95 %) monolayer coverage 1 h post-implantation and had greater endothelial coverage, smaller wall thickness, and more basement membrane after 9-week implantation, including a final week without anti-coagulation therapy, compared with contralateral non-seeded controls. These results support the use of autologous blood outgrowth endothelial cells as a viable source of endothelial cells for creating an endothelium with biological function on decellularized engineered allografts made from fibroblast-remodeled fibrin.

Original languageEnglish (US)
Pages (from-to)242-249
Number of pages8
JournalJournal of cardiovascular translational research
Volume7
Issue number2
DOIs
StatePublished - Mar 2014

Keywords

  • Endothelial cells
  • Tissue engineering
  • Vascular graft

Fingerprint Dive into the research topics of 'Blood outgrowth endothelial cells alter remodeling of completely biological engineered grafts implanted into the sheep femoral artery'. Together they form a unique fingerprint.

Cite this