Burn injury primes naive CD4+ T cells for an augmented T-helper 1 response

E. G. Kavanagh, J. L. Kelly, A. Lyons, C. C. Soberg, J. A. Mannick, J. A. Lederer, A. H. Harken, T. L. Pruett

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


Background. The mechanisms responsible for altered T-cell responses and cytokine production after injury are not well understood. We used T-cell receptor (TCR) transgenic mice to study burn injury effects on naive versus antigen-activated CD4+ T cells in vivo. Methods. One week after sham or burn injury, lymph node cells were prepared from TCR transgenic mice and stimulated with TCR transgene-specific antigens. T-cell proliferation was measured and culture supernatants were tested for interleukin-2 (IL-2), interferon-γ (IFN-γ), IL-4, and IL-10 by enzyme-linked immunosorbent assay (ELISA). Burn injury effects on antigen-activated T cells were studied by immunizing TCR transgenic or wild-type mice at the time of injury. Results. The antigen-stimulated proliferation of naive CD4+ T cells was unaffected by burn injury and no increase in T-helper 2 (Th2)-type cytokine production was observed. Instead, burn injury augmented IFN-γ production by naive CD4+ transgenic T cells, and IL-2 production was marginally reduced. Thus, burn injury primed naive T cells for an enhanced Th1-type response. In contrast, antigen-specific protiferation, IL-2, and IFN-γ production by T cells harvested from immunized wild-type mice were suppressed. Unexpectedly, high mortality was observed when burn-injured TCR transgenic mice were immunized. Conclusion. Our results show that burn injury has differential effects on naive and antigen-activated CD4+ T cells and can prime naive CD4+ T cells.

Original languageEnglish (US)
Pages (from-to)269-277
Number of pages9
Issue number2
StatePublished - Jan 1 1998

Fingerprint Dive into the research topics of 'Burn injury primes naive CD4+ T cells for an augmented T-helper 1 response'. Together they form a unique fingerprint.

Cite this