TY - JOUR
T1 - Caffeoyl shikimate esterase (CSE) is an enzyme in the lignin biosynthetic pathway in arabidopsis
AU - Vanholme, Ruben
AU - Cesarino, Igor
AU - Rataj, Katarzyna
AU - Xiao, Yuguo
AU - Sundin, Lisa
AU - Goeminne, Geert
AU - Kim, Hoon
AU - Cross, Joanna
AU - Morreel, Kris
AU - Araujo, Pedro
AU - Welsh, Lydia
AU - Haustraete, Jurgen
AU - McClellan, Christopher
AU - Vanholme, Bartel
AU - Ralph, John
AU - Simpson, Gordon G.
AU - Halpin, Claire
AU - Boerjan, Wout
PY - 2013
Y1 - 2013
N2 - Lignin is a major component of plant secondary cell walls. Here we describe caffeoyl shikimate esterase (CSE) as an enzyme central to the lignin biosynthetic pathway. Arabidopsis thaliana cse mutants deposit less lignin than do wild-type plants, and the remaining lignin is enriched in p-hydroxyphenyl units. Phenolic metabolite profiling identified accumulation of the lignin pathway intermediate caffeoyl shikimate in cse mutants as compared to caffeoyl shikimate levels in the wild type, suggesting caffeoyl shikimate as a substrate for CSE. Accordingly, recombinant CSE hydrolyzed caffeoyl shikimate into caffeate. Associated with the changes in lignin, the conversion of cellulose to glucose in cse mutants increased up to fourfold as compared to that in the wild type upon saccharification without pretreatment. Collectively, these data necessitate the revision of currently accepted models of the lignin biosynthetic pathway.
AB - Lignin is a major component of plant secondary cell walls. Here we describe caffeoyl shikimate esterase (CSE) as an enzyme central to the lignin biosynthetic pathway. Arabidopsis thaliana cse mutants deposit less lignin than do wild-type plants, and the remaining lignin is enriched in p-hydroxyphenyl units. Phenolic metabolite profiling identified accumulation of the lignin pathway intermediate caffeoyl shikimate in cse mutants as compared to caffeoyl shikimate levels in the wild type, suggesting caffeoyl shikimate as a substrate for CSE. Accordingly, recombinant CSE hydrolyzed caffeoyl shikimate into caffeate. Associated with the changes in lignin, the conversion of cellulose to glucose in cse mutants increased up to fourfold as compared to that in the wild type upon saccharification without pretreatment. Collectively, these data necessitate the revision of currently accepted models of the lignin biosynthetic pathway.
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U2 - 10.1126/science.1241602
DO - 10.1126/science.1241602
M3 - Article
C2 - 23950498
AN - SCOPUS:84883489184
SN - 0036-8075
VL - 341
SP - 1103
EP - 1106
JO - Science
JF - Science
IS - 6150
ER -