Calcineurin hydrolysis of para-nitrophenyl phosphorothioate

Donna J. Spannaus-Martin, Bruce L. Martin

Research output: Contribution to journalArticlepeer-review


para-Nitrophenyl phosphorothioate (pNPT) was hydrolyzed by calcineurin at initial rates slightly, but comparable to rates for para-nitrophenyl phosphate (pNPP). Kinetic characterization yielded higher estimates for both Km and Vmax compared to pNPP. Metal ion activation of phosphorothioate hydrolysis was more promiscuous. Unlike the hydrolysis of with pNPP, Ca 2+, Mg2+, and Ba2+ activated calcineurin as well as Mn2+.

Original languageEnglish (US)
Pages (from-to)149-155
Number of pages7
JournalProtein and Peptide Letters
Issue number2
StatePublished - Apr 1 2004


  • Calcineurin
  • Metal-activated enzyme
  • Substrate analog
  • Substrate specificity


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