Calcium isolation from large-volume human urine samples for 41Ca analysis by accelerator mass spectrometry

James J. Miller; Susanta K. Hui; George S. Jackson; Sara P. Clark; Jane Einstein; Connie M. Weaver; Maryka H. Bhattacharyya

doi:10.1016/j.apradiso.2013.04.012

Calcium isolation from large-volume human urine samples for ⁴¹Ca analysis by accelerator mass spectrometry

James J. Miller, Susanta K. Hui, George S. Jackson, Sara P. Clark, Jane Einstein, Connie M. Weaver, Maryka H. Bhattacharyya

Radiation Oncology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Calcium oxalate precipitation is the first step in preparation of biological samples for ⁴¹Ca analysis by accelerator mass spectrometry. A simplified protocol for large-volume human urine samples was characterized, with statistically significant increases in ion current and decreases in interference. This large-volume assay minimizes cost and effort and maximizes time after ⁴¹Ca administration during which human samples, collected over a lifetime, provide ⁴¹Ca:Ca ratios that are significantly above background.

Original language	English (US)
Pages (from-to)	57-61
Number of pages	5
Journal	Applied Radiation and Isotopes
Volume	78
DOIs	https://doi.org/10.1016/j.apradiso.2013.04.012
State	Published - Aug 2013

Keywords

Accelerator mass spectrometry
Biological samples
Ca analysis
Calcium oxalate
Human urine

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10.1016/j.apradiso.2013.04.012

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title = "Calcium isolation from large-volume human urine samples for 41Ca analysis by accelerator mass spectrometry",

abstract = "Calcium oxalate precipitation is the first step in preparation of biological samples for 41Ca analysis by accelerator mass spectrometry. A simplified protocol for large-volume human urine samples was characterized, with statistically significant increases in ion current and decreases in interference. This large-volume assay minimizes cost and effort and maximizes time after 41Ca administration during which human samples, collected over a lifetime, provide 41Ca:Ca ratios that are significantly above background.",

keywords = "Accelerator mass spectrometry, Biological samples, Ca analysis, Calcium oxalate, Human urine",

author = "Miller, {James J.} and Hui, {Susanta K.} and Jackson, {George S.} and Clark, {Sara P.} and Jane Einstein and Weaver, {Connie M.} and Bhattacharyya, {Maryka H.}",

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language = "English (US)",

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AU - Miller, James J.

AU - Hui, Susanta K.

AU - Jackson, George S.

AU - Clark, Sara P.

AU - Einstein, Jane

AU - Weaver, Connie M.

AU - Bhattacharyya, Maryka H.

PY - 2013/8

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AB - Calcium oxalate precipitation is the first step in preparation of biological samples for 41Ca analysis by accelerator mass spectrometry. A simplified protocol for large-volume human urine samples was characterized, with statistically significant increases in ion current and decreases in interference. This large-volume assay minimizes cost and effort and maximizes time after 41Ca administration during which human samples, collected over a lifetime, provide 41Ca:Ca ratios that are significantly above background.

KW - Accelerator mass spectrometry

KW - Biological samples

KW - Ca analysis

KW - Calcium oxalate

KW - Human urine

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