Calcium signals monitored from leopard frog optic tectum after the optic nerve has been selectively loaded with calcium sensitive dye

Elizabeth A. Dudkin; Paul Z. Myers; Jose A. Ramirez-Latorre; Edward R. Gruberg

doi:10.1016/S0304-3940(98)00870-2

Calcium signals monitored from leopard frog optic tectum after the optic nerve has been selectively loaded with calcium sensitive dye

Elizabeth A. Dudkin, Paul Z. Myers, Jose A. Ramirez-Latorre, Edward R. Gruberg

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5 Scopus citations

Abstract

We loaded adult leopard frog optic nerves with the calcium-sensitive dye Calcium Green-1 3000 mw dextran conjugate. The dye was transported to the optic tectum in approximately 6 days and selectively labeled optic nerve terminals as seen with confocal microscopy. Viewed with an intensified CCD system, electrical stimulation of the optic nerve in vitro increases. Calcium Green-1 fluorescence significantly. With increasing number of pulses in pulse trains there was increased presynaptic facilitation as measured by increased fluorescence. Addition of nicotine to the bathing solution increased baseline fluorescence. These results suggest that Calcium Green-1 dextran conjugate can be actively transported in adult nerve fibers over a significant distance and is retained in presynaptic terminals in a form that allows monitoring or presynaptic calcium levels.

Original language	English (US)
Pages (from-to)	124-126
Number of pages	3
Journal	Neuroscience Letters
Volume	258
Issue number	2
DOIs	https://doi.org/10.1016/S0304-3940(98)00870-2
State	Published - Dec 18 1998
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by NIH grant EY 04366, NSF grant DBI9601599 and Temple University. We thank Michael O'Donovan for useful advice and Christopher Neeb for help with the graphics.

Keywords

Calcium green
Leopard frog
Optic nerve
Optic tectum
Presynaptic facilitation

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title = "Calcium signals monitored from leopard frog optic tectum after the optic nerve has been selectively loaded with calcium sensitive dye",

abstract = "We loaded adult leopard frog optic nerves with the calcium-sensitive dye Calcium Green-1 3000 mw dextran conjugate. The dye was transported to the optic tectum in approximately 6 days and selectively labeled optic nerve terminals as seen with confocal microscopy. Viewed with an intensified CCD system, electrical stimulation of the optic nerve in vitro increases. Calcium Green-1 fluorescence significantly. With increasing number of pulses in pulse trains there was increased presynaptic facilitation as measured by increased fluorescence. Addition of nicotine to the bathing solution increased baseline fluorescence. These results suggest that Calcium Green-1 dextran conjugate can be actively transported in adult nerve fibers over a significant distance and is retained in presynaptic terminals in a form that allows monitoring or presynaptic calcium levels.",

keywords = "Calcium green, Leopard frog, Optic nerve, Optic tectum, Presynaptic facilitation",

author = "Dudkin, {Elizabeth A.} and Myers, {Paul Z.} and Ramirez-Latorre, {Jose A.} and Gruberg, {Edward R.}",

note = "Funding Information: This work was supported by NIH grant EY 04366, NSF grant DBI9601599 and Temple University. We thank Michael O'Donovan for useful advice and Christopher Neeb for help with the graphics.",

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AU - Dudkin, Elizabeth A.

AU - Myers, Paul Z.

AU - Ramirez-Latorre, Jose A.

AU - Gruberg, Edward R.

N1 - Funding Information: This work was supported by NIH grant EY 04366, NSF grant DBI9601599 and Temple University. We thank Michael O'Donovan for useful advice and Christopher Neeb for help with the graphics.

PY - 1998/12/18

Y1 - 1998/12/18

N2 - We loaded adult leopard frog optic nerves with the calcium-sensitive dye Calcium Green-1 3000 mw dextran conjugate. The dye was transported to the optic tectum in approximately 6 days and selectively labeled optic nerve terminals as seen with confocal microscopy. Viewed with an intensified CCD system, electrical stimulation of the optic nerve in vitro increases. Calcium Green-1 fluorescence significantly. With increasing number of pulses in pulse trains there was increased presynaptic facilitation as measured by increased fluorescence. Addition of nicotine to the bathing solution increased baseline fluorescence. These results suggest that Calcium Green-1 dextran conjugate can be actively transported in adult nerve fibers over a significant distance and is retained in presynaptic terminals in a form that allows monitoring or presynaptic calcium levels.

AB - We loaded adult leopard frog optic nerves with the calcium-sensitive dye Calcium Green-1 3000 mw dextran conjugate. The dye was transported to the optic tectum in approximately 6 days and selectively labeled optic nerve terminals as seen with confocal microscopy. Viewed with an intensified CCD system, electrical stimulation of the optic nerve in vitro increases. Calcium Green-1 fluorescence significantly. With increasing number of pulses in pulse trains there was increased presynaptic facilitation as measured by increased fluorescence. Addition of nicotine to the bathing solution increased baseline fluorescence. These results suggest that Calcium Green-1 dextran conjugate can be actively transported in adult nerve fibers over a significant distance and is retained in presynaptic terminals in a form that allows monitoring or presynaptic calcium levels.

KW - Calcium green

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KW - Optic nerve

KW - Optic tectum

KW - Presynaptic facilitation

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JO - Neuroscience Letters

JF - Neuroscience Letters

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ER -

Calcium signals monitored from leopard frog optic tectum after the optic nerve has been selectively loaded with calcium sensitive dye

Abstract

Bibliographical note

Keywords

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