Calcium uptake and release by skeletal-muscle mitochondria

James R. Mickelson; B. Bruce Marsh

doi:10.1016/0143-4160(80)90025-1

Calcium uptake and release by skeletal-muscle mitochondria

James R. Mickelson, B. Bruce Marsh

Veterinary and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

THE EGTA - ruthenium red quench technique was used to obtain initial-velocity plots of Ca²⁺ uptake by skeletal-muscle mitochondria. The K_m was 5 μM and the Hill coefficient 1.9 at both 0° and 10°C. Inorganic phosphate stimulated and Mg²⁺ inhibited initial rates of transport. In experiments on Ca²⁺ release, the Na⁺ Ca²⁺ exchange was demonstrated. Factors influencing Ca²⁺ release during anaerobiosis include phosphate concentration and extent of Ca²⁺ loading. The results are discussed in relation to the possible participation of mitochondria in the calcium-ion regulation of muscle.

Original language	English (US)
Pages (from-to)	119-128
Number of pages	10
Journal	Cell Calcium
Volume	1
Issue number	2
DOIs	https://doi.org/10.1016/0143-4160(80)90025-1
State	Published - Mar 1980

Bibliographical note

Funding Information:
This research was supported by the College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin. We are grateful to Dr. Marion Greaser for helpful advice and constructive criticism, and to Oscar Mayer and Company for the supply of muscles. Muscle Biology Manuscript #l40.

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title = "Calcium uptake and release by skeletal-muscle mitochondria",

abstract = "THE EGTA - ruthenium red quench technique was used to obtain initial-velocity plots of Ca2+ uptake by skeletal-muscle mitochondria. The Km was 5 μM and the Hill coefficient 1.9 at both 0° and 10°C. Inorganic phosphate stimulated and Mg2+ inhibited initial rates of transport. In experiments on Ca2+ release, the Na+ Ca2+ exchange was demonstrated. Factors influencing Ca2+ release during anaerobiosis include phosphate concentration and extent of Ca2+ loading. The results are discussed in relation to the possible participation of mitochondria in the calcium-ion regulation of muscle.",

author = "Mickelson, {James R.} and Marsh, {B. Bruce}",

note = "Funding Information: This research was supported by the College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin. We are grateful to Dr. Marion Greaser for helpful advice and constructive criticism, and to Oscar Mayer and Company for the supply of muscles. Muscle Biology Manuscript #l40.",

year = "1980",

month = mar,

doi = "10.1016/0143-4160(80)90025-1",

language = "English (US)",

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journal = "Cell Calcium",

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T1 - Calcium uptake and release by skeletal-muscle mitochondria

AU - Mickelson, James R.

AU - Marsh, B. Bruce

N1 - Funding Information: This research was supported by the College of Agricultural and Life Sciences, University of Wisconsin, Madison, Wisconsin. We are grateful to Dr. Marion Greaser for helpful advice and constructive criticism, and to Oscar Mayer and Company for the supply of muscles. Muscle Biology Manuscript #l40.

PY - 1980/3

Y1 - 1980/3

N2 - THE EGTA - ruthenium red quench technique was used to obtain initial-velocity plots of Ca2+ uptake by skeletal-muscle mitochondria. The Km was 5 μM and the Hill coefficient 1.9 at both 0° and 10°C. Inorganic phosphate stimulated and Mg2+ inhibited initial rates of transport. In experiments on Ca2+ release, the Na+ Ca2+ exchange was demonstrated. Factors influencing Ca2+ release during anaerobiosis include phosphate concentration and extent of Ca2+ loading. The results are discussed in relation to the possible participation of mitochondria in the calcium-ion regulation of muscle.

AB - THE EGTA - ruthenium red quench technique was used to obtain initial-velocity plots of Ca2+ uptake by skeletal-muscle mitochondria. The Km was 5 μM and the Hill coefficient 1.9 at both 0° and 10°C. Inorganic phosphate stimulated and Mg2+ inhibited initial rates of transport. In experiments on Ca2+ release, the Na+ Ca2+ exchange was demonstrated. Factors influencing Ca2+ release during anaerobiosis include phosphate concentration and extent of Ca2+ loading. The results are discussed in relation to the possible participation of mitochondria in the calcium-ion regulation of muscle.

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JO - Cell Calcium

JF - Cell Calcium

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Calcium uptake and release by skeletal-muscle mitochondria

Abstract

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