Callus formation and plant regeneration from somatic embryos of oat (Avena sativa L.)

P. Bregitzer, W. R. Bushnell, H. W. Rines, D. A. Somers

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9 Scopus citations

Abstract

Globular-stage somatic embryos were isolated by vortexing friable, embryogenic callus of oat (Avena sativa L.) followed by fractionation based on size. Somatic embryos were most frequently found in the 300-380 μm size fraction. Friable, embryogenic callus was reinitiated from 55% of isolated somatic embryos. Fertile plants were regenerated from 22% of isolated somatic embryos. Reinitiation of callus from somatic embryos and growth of friable, embryogenic callus was inhibited by the selective agents G418 and methotrexate. These results suggest that somatic embryos isolated from friable, embryogenic callus of oat may be useful totipotent targets for particle acceleration-mediated transformation.

Original languageEnglish (US)
Pages (from-to)243-246
Number of pages4
JournalPlant Cell Reports
Volume10
Issue number5
DOIs
StatePublished - Aug 1 1991

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