Can deliberately incomplete gene sample augmentation improve a phylogeny estimate for the advanced moths and butterflies (hexapoda: Lepidoptera)?

Soowon Cho, Andreas Zwick, Jerome C. Regier, Charles Mitter, Michael P. Cummings, Jianxiu Yao, Zaile Du, Hong Zhao, Akito Y. Kawahara, Susan Weller, Donald R. Davis, Joaquin Baixeras, John W. Brown, Cynthia Parr

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73 Scopus citations

Abstract

This paper addresses the question of whether one can economically improve the robustness of a molecular phylogeny estimate by increasing gene sampling in only a subset of taxa, without having the analysis invalidated by artifacts arising from large blocks of missing data. Our case study stems from an ongoing effort to resolve poorly understood deeper relationships in the large clade Ditrysia (> 150,000 species) of the insect order Lepidoptera (butterflies and moths). Seeking to remedy the overall weak support for deeper divergences in an initial study based on five nuclear genes (6.6 kb) in 123 exemplars, we nearly tripled the total gene sample (to 26 genes, 18.4 kb) but only in a third (41) of the taxa. The resulting partially augmented data matrix (45% intentionally missing data) consistently increased bootstrap support for groupings previously identified in the five-gene (nearly) complete matrix, while introducing no contradictory groupings of the kind that missing data have been predicted to produce. Our results add to growing evidence that data sets differing substantially in gene and taxon sampling can often be safely and profitably combined. The strongest overall support for nodes above the family level came from including all nucleotide changes, while partitioning sites into sets undergoing mostly nonsynonymous versus mostly synonymous change. In contrast, support for the deepest node for which any persuasive molecular evidence has yet emerged (78-85% bootstrap) was weak or nonexistent unless synonymous change was entirely excluded, a result plausibly attributed to compositional heterogeneity. This node (Gelechioidea + Apoditrysia), tentatively proposed by previous authors on the basis of four morphological synapomorphies, is the first major subset of ditrysian superfamilies to receive strong statistical support in any phylogenetic study. A "more-genes-only" data set (41 taxa×26 genes) also gave strong signal for a second deep grouping (Macrolepidoptera) that was obscured, but not strongly contradicted, in more taxon-rich analyses.

Original languageEnglish (US)
Pages (from-to)782-796
Number of pages15
JournalSystematic Biology
Volume60
Issue number6
DOIs
StatePublished - Dec 2011

Bibliographical note

Funding Information:
FUNDING Financial support was provided by the US National Science Foundation’s Assembling the Tree of Life program, award numbers 0531626 and 0531769; the Spanish Government (Ministerio de Ciencia e Innovación) (CGL2008-00605 to J.B.); US National Science Foundation (DEB 0515699 to D. H. Janzen).

Keywords

  • Ditrysia
  • Hexapoda
  • Lepidoptera
  • gene sampling
  • missing data
  • molecular phylogenetics
  • nuclear genes
  • taxon sampling

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