Carbofuran-induced oxidative stress in mammalian brain

Devendra K. Rai, Bechan Sharma

Research output: Contribution to journalArticlepeer-review

73 Scopus citations

Abstract

Chronic exposure to carbofuran, a carbamate pesticide, via oral administration has been reported to generate reactive oxygen species (ROS) in rat brain. However, information regarding the effect of short-term intraperitoneal (i.p.) carbofuran intoxication on oxidative stress is lacking. In the present study, the effect of carbofuran on oxidative indices in brain of Wistar rats has been determined by exposing the animals to three subacute concentrations (0.2, 0.4 and 0.8 mg/kg body weight) equivalent to 10, 20, and 40%, respectively, of its LD50 (i.p.) for 24 h. Rat liver has been used as a positive control. The results demonstrated that carbofuran treatment at the 3 concentrations tested caused significant increase in lipid peroxidation (LPO) by 12.50, 34.38, and 59.38%, respectively. The increased oxidative stress at same pesticide concentrations significantly induced activities of antioxidant enzymes such as superoxide dismutase (SOD) and catalase in rat brain; the impact on catalase being more marked only at high-pesticide doses (0.4 and 0.8 mg/kg body weight). Carbofuran also caused reduction in protein content of rat tissues tested. Rat brain was more severely affected by carbofuran than liver. The results clearly demonstrated that i.p. administration of carbofuran accelerated oxidative stress in rat brain in a dose-dependent manner.

Original languageEnglish (US)
Pages (from-to)66-71
Number of pages6
JournalMolecular Biotechnology
Volume37
Issue number1
DOIs
StatePublished - Sep 1 2007

Keywords

  • Brain
  • Carbofuran
  • Catalase
  • Lipid peroxidation
  • Liver
  • Oxidative indices
  • Pesticide
  • Rats
  • Superoxide dismutase

Fingerprint Dive into the research topics of 'Carbofuran-induced oxidative stress in mammalian brain'. Together they form a unique fingerprint.

Cite this