The effect of utilizable carbon sources on the production of cephem antibiotics by Streptomyces clavuligerus has been studied. The pattern of utilizable carbon sources was found to be very restricted. Glycerol, maltose and starch supported the most extensive growth. Increasing the initial concentrations of carbon sources decreased both the volumetric and the specific production of cephems. A resting cell system was adopted for this study. Linear production of cephems continued for 4-7 h. The production rate of the resting cell system was higher with mycelia harvested at an early exponential stage than with those harvested at a late stage of growth. Addition of carbon source to the resting cell system decreased the production rate of cephems. This suppressive effect was prevented by the addition of chloramphenicol (or streptomycin) although uptake of carbon source was not inhibited by such a protein synthesis inhibitor.
Bibliographical noteFunding Information:
We thank Thomas G. Pridham of the US Department of Agriculture and Yair Aharonowitz of Tel Aviv University for cultures. Dr Aharonowitz, as well as Nadine A. Solomon and Jacqueline M. Piret, are acknowledged for advice and help. We also appreciate the assistance of Norbert Neuss and S. M. Lawrence of the Lilly Research Laboratories. Partial support for this project was provided by the National Science Foundation (DAR 79-19493) and the National Institutes of Health (S RO1 AI16640). A. F. Brafia was supported by a fellowship from the Fundaci6n Juan March of Spain.
- Antibiotic production
- Streptomyces clavuligerus
- carbon source regulation