Cardioprotective molecules are enriched in beating cardiomyocytes derived from human embryonic stem cells

Jung Il Chae, Jumi Kim, Mi Young Son, Young Joo Jeon, Dong Wook Kim, Hye Eun Kim, Min Ji Lee, Joohyun Ryu, Byoung Chul Park, Dong Seok Lee, Kang Seok Seo, Hak Kyo Lee, Nag Jin Choi, Yong Kook Kang, Hyung Min Chung

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Background: Cardiomyocytes derived from human embryonic stem cells (hESC-CMs) have attracted attention because of their cardiac regenerative potential in vivo. Differentiated CMs can be distinguished into two different phenotypic populations: beating and non-beating CMs. A thorough understanding of the different molecular conditions of beating and non-beating CMs would provide valuable information for other potential applications and cell therapy. Methods: In this study, we generated a comparative protein profiles using proteomic analysis and western blotting, to compare the specific protein expression patterns of beating and non-beating hESC-CMs. Results: Abundantly 72 upregulated proteins are involved in different biological processes such as stimulus response, cellular catabolism and cell motility. Among these proteins, such as HSPs and other antioxidant molecules, are known to be proteins that potentially play an important role in cardioprotection through the enhancement of cell survival in hypoxic and ischemic conditions present in the injured heart. Conclusion: As a first step toward understanding the different molecular conditions of beating and non-beating hESC-CMs, we sought to study their differential expression patterns and discuss their relevance to in vivo functioning in cardiac injury repair. Thus, the results of this study could provide further evidence supporting a cardiac regenerative approach using an optimized cell source derived from hESCs.

Original languageEnglish (US)
Pages (from-to)341-354
Number of pages14
JournalInternational Journal of Cardiology
Volume165
Issue number2
DOIs
StatePublished - May 10 2013

Bibliographical note

Funding Information:
This work was supported by the NRF ( 2012-0006146 ) and the NRF-SRC ( 2011-0030769 ), by a grant ( 10033642 ) from the Industry Sources Development Project funded by the Ministry of Knowledge Economy, Republic of Korea , by the Next-Generation BioGreen 21 Program ( PJ008116 ), Rural Development Administration, Republic of Korea , and by a grant ( 2010-0021532 ) from the basic Science Research Program . The authors of this manuscript have certified that they comply with the Principles of Ethical Publishing in the International Journal of Cardiology.

Keywords

  • Cardiomyocytes
  • Embryonic body
  • Human embryonic stem cell
  • Proteomic analysis

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