Catecholaminergic, cholinergic and peptidergic innervation of gut-associated lymphoid tissue in porcine jejunum and ileum

Anjali Kulkarni-Narla, Alvin J. Beitz, David R. Brown

Research output: Contribution to journalArticlepeer-review

62 Scopus citations


With its abundance of neurons and immunocytes, the gut is a potentially important site for the study of the interaction between the nervous and immune systems. Using immunohistochemical techniques, we tested the hypothesis that gut-associated lymphoid tissue in the porcine small intestine might receive catecholaminergic, cholinergic and peptidergic innervation. Antibodies against protein gene product (PGP) 9.5 were employed to detect neuronal membranes; antibodies against tyrosine hydroxylase (TH), type 2 vesicular monoamine transporter (VMAT-2) and choline acetyltransferase (ChAT) were used to detect catecholaminergic and cholinergic neurons; and antibodies to neuromedin U-8 (NMU-8), substance P (SP) and vasoactive intestinal peptide (VIP) were also used. PGP9.5-immunoreactive nerve fibers were observed between jejunal Peyer's patch (PP) follicles and in submucosal ganglia localized at the base of continuous ileal PP. Many ChAT-positive and a few TH-/VMAT-2-immunoreactive neurons or axons adjacent to jejunal and ileal PP were observed. Neurons and fibers from ganglia situated between or at the base of PP follicles manifested robust immunoreactivities to VIP and NMU-8; relatively less SP immunoreactivity was observed at these locations. All neuromedin-U 8-positive neurons observed exhibited immunoreactivity to ChAT as did some VIP-positive neurons. The specific chemical coding of enteric neurons in close apposition to jejunal and ileal PP and the differential localization of neuropeptides within the jejunal and ileal PP are indicative of neuroimmunomodulation at these sites.

Original languageEnglish (US)
Pages (from-to)275-286
Number of pages12
JournalCell and Tissue Research
Issue number2
StatePublished - 1999

Bibliographical note

Funding Information:
This investigation was supported by NIH grant DA-10200. A.K.-N. is a postdoctoral trainee supported by NIH Psychoneuroimmunology Training Grant DA-07239.


  • Choline acetyltransferase
  • Neuroimmunology
  • Neuromedin U-8
  • Peyer's patch
  • Pig
  • Substance P
  • Type 2 vesicular monoamine transporter
  • Tyrosine hydroxylase
  • Vasoactive intestinal peptide


Dive into the research topics of 'Catecholaminergic, cholinergic and peptidergic innervation of gut-associated lymphoid tissue in porcine jejunum and ileum'. Together they form a unique fingerprint.

Cite this