CD5 maintains tolerance in anergic B cells

Keli L Hippen, Lina E. Tze, Timothy W. Behrens

Research output: Contribution to journalArticlepeer-review

169 Scopus citations

Abstract

Clonal anergy of autoreactive B cells is a key mechanism regulating tolerance. Here, we show that anergic B cells express significant surface levels of CD5, a molecule normally found on T cells and a subset of B-1 cells. Breeding of the hen egg lysozyme (HEL) transgenic model for B cell anergy onto the CD5 null background resulted in a spontaneous loss orb cell tolerance in vivo. Evidence for this included elevated levels of anti-HEL immunoglobulin M (IgM) antibodies in the serum of CD5(-/-) mice transgenic for both an HEL-specific B cell receptor (BCR) and soluble lysozyme. 'Anergic' B cells lacking CD5 also showed enhanced proliferative responses in vitro and elevated intracellular Ca2+ levels at rest and after IgM cross-linking. These data support the hypothesis that CD5 negatively regulates Ig receptor signaling in anergic B cells and functions to inhibit autoimmune B cell responses.

Original languageEnglish (US)
Pages (from-to)883-889
Number of pages7
JournalJournal of Experimental Medicine
Volume191
Issue number5
DOIs
StatePublished - Mar 6 2000

Keywords

  • Anergy
  • B cell
  • CD5
  • Hen egg lysozyme
  • Signal transduction

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