CE-SELEX: Isolating aptamers using capillary electrophoresis

Renee K. Mosing, Michael T. Bowser

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Since their discovery in 1990, aptamers have gained increasing attention due to their high affinity and specificity for a wide range of target molecules.1-5 While aptamer applications are growing exponentially, the time and difficulty of the process used to isolate aptamers are limiting widespread adoption. Recently, the high resolving power of capillary electrophoresis (CE) has been used to select high-affinity aptamers.6,7 This offers a number of advantages over conventional selection protocols due to the dramatic enrichment rate and selection stringency made possible by CE. These advantages result in a significantly faster and simpler process allowing high-affinity aptamers to be obtained in several days rather than the weeks to months typical of pre-existing procedures. This chapter discusses the significance of CE-SELEX and describes details of the selection process.

Original languageEnglish (US)
Title of host publicationHandbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition
PublisherCRC Press
Pages825-840
Number of pages16
ISBN (Electronic)9781420004953
ISBN (Print)9780849333293
StatePublished - Jan 1 2007

Fingerprint Dive into the research topics of 'CE-SELEX: Isolating aptamers using capillary electrophoresis'. Together they form a unique fingerprint.

Cite this