TY - JOUR
T1 - Cell line development for biomanufacturing processes
T2 - recent advances and an outlook
AU - Le, Huong
AU - Vishwanathan, Nandita
AU - Jacob, Nitya M.
AU - Gadgil, Mugdha
AU - Hu, Wei Shou
PY - 2015/8/1
Y1 - 2015/8/1
N2 - At the core of a biomanufacturing process for recombinant proteins is the production cell line. It influences the productivity and product quality. Its characteristics also dictate process development, as the process is optimized to complement the producing cell to achieve the target productivity and quality. Advances in the past decade, from vector design to cell line screening, have greatly expanded our capability to attain producing cell lines with certain desired traits. Increasing availability of genomic and transcriptomic resources for industrially important cell lines coupled with advances in genome editing technology have opened new avenues for cell line development. These developments are poised to help biosimilar manufacturing, which requires targeting pre-defined product quality attributes, e.g., glycoform, to match the innovator’s range. This review summarizes recent advances and discusses future possibilities in this area.
AB - At the core of a biomanufacturing process for recombinant proteins is the production cell line. It influences the productivity and product quality. Its characteristics also dictate process development, as the process is optimized to complement the producing cell to achieve the target productivity and quality. Advances in the past decade, from vector design to cell line screening, have greatly expanded our capability to attain producing cell lines with certain desired traits. Increasing availability of genomic and transcriptomic resources for industrially important cell lines coupled with advances in genome editing technology have opened new avenues for cell line development. These developments are poised to help biosimilar manufacturing, which requires targeting pre-defined product quality attributes, e.g., glycoform, to match the innovator’s range. This review summarizes recent advances and discusses future possibilities in this area.
KW - Biomanufacturing
KW - Biosimilars
KW - CHO cells genomics
KW - Cell line development
KW - Genome editing
KW - Transcriptomics
UR - http://www.scopus.com/inward/record.url?scp=84933676693&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84933676693&partnerID=8YFLogxK
U2 - 10.1007/s10529-015-1843-z
DO - 10.1007/s10529-015-1843-z
M3 - Review article
C2 - 25971160
AN - SCOPUS:84933676693
SN - 0141-5492
VL - 37
SP - 1553
EP - 1564
JO - Biotechnology Letters
JF - Biotechnology Letters
IS - 8
ER -