Cell-type specificity of anti-CD45 autoantibodies in systemic lupus erythematosus

Jan Czyzyk, Philip Fernsten, Melody Shaw, John B. Winfield

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Objective. To determine the specificity of anti-CD45 autoantibodies in systemic lupus erythematosus (SLE) for native CD45 and for CD45 expressed by T cells and B cells, and at different stages of cellular activation. Methods. CD45 purified from different types of lymphocytes was examined by immunoblotting with sera from patients with SLE. Indirect immunofluorescence experiments were performed with purified anti-CD45 autoantibodies. Results. IgM anti-CD45 autoantibodies in SLE recognize native CD45 expressed on the surface membrane of viable lymphocytes and CD45 purified from activated peripheral T cells and certain T cell lines, but not CD45 purified from B cells or resting peripheral T cells. The presence or absence of reactivity is independent of the individual isoforms expressed. Conclusion. Recognition of CD45 by IgM anti-lymphocyte autoantibodies in SLE varies with the lineage and state of activation of the lymphocyte target. This pattern of reactivity is consistent with autoantibody specificities involving CD45 glycoforms, rather than CD45 isoforms.

Original languageEnglish (US)
Pages (from-to)592-599
Number of pages8
JournalArthritis and rheumatism
Volume39
Issue number4
DOIs
StatePublished - Apr 1996
Externally publishedYes

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