TY - JOUR
T1 - Cellular immune responses to four doses of percutaneous bacille calmetteguerin in healthy adults
AU - Lowry, Philip W.
AU - Ludwig, Teresa S.
AU - Adams, Jeffrey A.
AU - Fitzpatrick, Michael L.
AU - Grant, Suzanne M.
AU - Andrle, Greg A.
AU - Offerdahl, Matthew R.
AU - Cho, Sang Nae
AU - Jacobs, David R.
PY - 1998
Y1 - 1998
N2 - To explore the hypothesis that low-dose immunization might induce preferential Th1 cell immunity, 76 adults were vaccinated with one of four doses of bacille Calmette-Guerin (BCG): The doses contained very low (1.6 x 105 cfu), low (3.2 x l06 cfu), standard (1.6 x 108 cfu), or high (3.2 X 108 cfu) levels of BCG. Delayed-type hypersensitivity responses occurred 8 weeks after vaccination in 10% of persons given very low or low doses of BCG, compared with 95% and 100% of persons given standard or high doses, respectively. Lymphoproliferative responses, which were increased only for high-dose vaccinees, peaked 2 weeks after vaccination and were directed chiefly against Mycobacterium tuberculosis-secreted proteins, particularly the antigen 85 complex. Significant increases in mycobacteria-specific interferon-γ expression were present 16 weeks after vaccination only for persons given standard or high doses of BCG. Percutaneous BCG appears capable of inducing a temporary Th1-like immune response, but standard or higher dosages are required.
AB - To explore the hypothesis that low-dose immunization might induce preferential Th1 cell immunity, 76 adults were vaccinated with one of four doses of bacille Calmette-Guerin (BCG): The doses contained very low (1.6 x 105 cfu), low (3.2 x l06 cfu), standard (1.6 x 108 cfu), or high (3.2 X 108 cfu) levels of BCG. Delayed-type hypersensitivity responses occurred 8 weeks after vaccination in 10% of persons given very low or low doses of BCG, compared with 95% and 100% of persons given standard or high doses, respectively. Lymphoproliferative responses, which were increased only for high-dose vaccinees, peaked 2 weeks after vaccination and were directed chiefly against Mycobacterium tuberculosis-secreted proteins, particularly the antigen 85 complex. Significant increases in mycobacteria-specific interferon-γ expression were present 16 weeks after vaccination only for persons given standard or high doses of BCG. Percutaneous BCG appears capable of inducing a temporary Th1-like immune response, but standard or higher dosages are required.
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U2 - 10.1086/515614
DO - 10.1086/515614
M3 - Article
C2 - 9652433
AN - SCOPUS:0031817336
SN - 0022-1899
VL - 178
SP - 138
EP - 146
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 1
ER -