TY - JOUR
T1 - Cellular interactions in the generation of cytolytic T lymphocyte responses Analysis of the helper T cell pathway
AU - Weinberger, Ofra
AU - Herrmann, Steven
AU - Mescher, Matthew F.
AU - Benacerraf, Baruj
AU - Burakoff, Steven J.
PY - 1981
Y1 - 1981
N2 - Murine splenic lymphocytes exhibit a requirement for helper T cells for the induction of a cytolytic T lymphocyte response to suboptimal doses of allogeneic cells, membranes from allogeneic cells, or purified H‐2 antigen in liposomes. The conditions where a requirement for help is apparent are the same conditions where a dependence on splenic adherent cells (SAC) has been demonstrated (Weinberger, O. et al., Proc. Natl. Acad. Sci. USA 1980. 77: 6091). Help can be provided in the form of primed, radioresistant, Ly‐1+ spleen or lymph node cells or helper factor (interleukin 2, IL‐2). A factor generated from phytohemagglutinin‐stimulated human lymphocytes, when added to culture in the presence of antigen, bypassed the requirement for Ia+ SAC and helper cells. IL‐2 reconstituted the response to H‐2Kk in liposomes in cultures depleted of SAC, strongly suggesting that the helper cell must see antigen re‐expressed by an antigen‐presenting cell, whereas the prekiller does not. IL‐2 could be generated by culturing Ly‐1+ murine spleen cells with H‐2Kk pulsed on SAC.
AB - Murine splenic lymphocytes exhibit a requirement for helper T cells for the induction of a cytolytic T lymphocyte response to suboptimal doses of allogeneic cells, membranes from allogeneic cells, or purified H‐2 antigen in liposomes. The conditions where a requirement for help is apparent are the same conditions where a dependence on splenic adherent cells (SAC) has been demonstrated (Weinberger, O. et al., Proc. Natl. Acad. Sci. USA 1980. 77: 6091). Help can be provided in the form of primed, radioresistant, Ly‐1+ spleen or lymph node cells or helper factor (interleukin 2, IL‐2). A factor generated from phytohemagglutinin‐stimulated human lymphocytes, when added to culture in the presence of antigen, bypassed the requirement for Ia+ SAC and helper cells. IL‐2 reconstituted the response to H‐2Kk in liposomes in cultures depleted of SAC, strongly suggesting that the helper cell must see antigen re‐expressed by an antigen‐presenting cell, whereas the prekiller does not. IL‐2 could be generated by culturing Ly‐1+ murine spleen cells with H‐2Kk pulsed on SAC.
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U2 - 10.1002/eji.1830110511
DO - 10.1002/eji.1830110511
M3 - Article
C2 - 6973476
AN - SCOPUS:0019415601
SN - 0014-2980
VL - 11
SP - 405
EP - 411
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 5
ER -