TY - JOUR
T1 - Cellular pathogenesis of porcine circovirus type 2 infection
AU - Dvorak, Cheryl M.T.
AU - Puvanendiran, Sumathy
AU - Murtaugh, Michael P.
PY - 2013/6/1
Y1 - 2013/6/1
N2 - A porcine cell line, VR1BL, was characterized for high titer growth of PCV2.•PCV2 grows to higher titers in VR1BL cells, than in PK-15 cells.•This cell line provides an in vitro model of acute versus persistent infection that replicates in vivo behavior of PCV2.•High MOI infection induces apoptosis-mediated cell death. Porcine circovirus associated disease (PCVAD) and the associated histological lesions are thought to appear due to an increase in the amount of porcine circovirus type 2 (PCV2) present in an infected animal. However, examination of the cellular and molecular pathogenesis of PCVAD is complicated by the lack of a consistent cell culture model that replicates the animal phenotypes of persistent, asymptomatic infection, and acute, pathological disease typified by lymphocyte depletion. The porcine fetal retina cell line, VR1BL, shows a high permissiveness to PCV2 infection, 40 times higher than the alternative PK15 culture model, allowing for high titer viral production, with PCV2b growth higher than PCV2a growth. Cytopathic effect due to apoptosis is observed after challenge with high amounts of PCV2, but at low levels, infection is maintained in passaged cells. Thus, VR1BL cells may be used as a model system to examine both acute viral pathogenesis and cellular innate defense, as well as persistent PCV2 infection.
AB - A porcine cell line, VR1BL, was characterized for high titer growth of PCV2.•PCV2 grows to higher titers in VR1BL cells, than in PK-15 cells.•This cell line provides an in vitro model of acute versus persistent infection that replicates in vivo behavior of PCV2.•High MOI infection induces apoptosis-mediated cell death. Porcine circovirus associated disease (PCVAD) and the associated histological lesions are thought to appear due to an increase in the amount of porcine circovirus type 2 (PCV2) present in an infected animal. However, examination of the cellular and molecular pathogenesis of PCVAD is complicated by the lack of a consistent cell culture model that replicates the animal phenotypes of persistent, asymptomatic infection, and acute, pathological disease typified by lymphocyte depletion. The porcine fetal retina cell line, VR1BL, shows a high permissiveness to PCV2 infection, 40 times higher than the alternative PK15 culture model, allowing for high titer viral production, with PCV2b growth higher than PCV2a growth. Cytopathic effect due to apoptosis is observed after challenge with high amounts of PCV2, but at low levels, infection is maintained in passaged cells. Thus, VR1BL cells may be used as a model system to examine both acute viral pathogenesis and cellular innate defense, as well as persistent PCV2 infection.
KW - Cell culture
KW - PCV2
KW - Porcine circovirus 2
KW - Replication kinetics
KW - Swine
KW - Viral growth
UR - http://www.scopus.com/inward/record.url?scp=84877600516&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84877600516&partnerID=8YFLogxK
U2 - 10.1016/j.virusres.2013.03.001
DO - 10.1016/j.virusres.2013.03.001
M3 - Article
C2 - 23499996
AN - SCOPUS:84877600516
SN - 0168-1702
VL - 174
SP - 60
EP - 68
JO - Virus research
JF - Virus research
IS - 1-2
ER -