TY - JOUR
T1 - Central cell-derived peptides regulate early embryo patterning in flowering plants
AU - Costa, Liliana M.
AU - Marshall, Eleanor
AU - Tesfaye, Mesfin
AU - Silverstein, Kevin A.T.
AU - Mori, Masashi
AU - Umetsu, Yoshitaka
AU - Otterbach, Sophie L.
AU - Papareddy, Ranjith
AU - Dickinson, Hugh G.
AU - Boutiller, Kim
AU - VandenBosch, Kathryn A.
AU - Ohki, Shinya
AU - Gutierrez-Marcos, José F.
PY - 2014
Y1 - 2014
N2 - Plant embryogenesis initiates with the establishment of an apical-basal axis; however, the molecular mechanisms accompanying this early event remain unclear. Here, we show that a small cysteine-rich peptide family is required for formation of the zygotic basal cell lineage and proembryo patterning in Arabidopsis. EMBRYO SURROUNDING FACTOR 1 (ESF1) peptides accumulate before fertilization in central cell gametes and thereafter in embryo-surrounding endosperm cells. Biochemical and structural analyses revealed cleavage of ESF1 propeptides to form biologically active mature peptides. Further, these peptides act in a non - cell-autonomous manner and synergistically with the receptor-like kinase SHORT SUSPENSOR to promote suspensor elongation through the YODA mitogen-activated protein kinase pathway. Our findings demonstrate that the second female gamete and its sexually derived endosperm regulate early embryonic patterning in flowering plants.
AB - Plant embryogenesis initiates with the establishment of an apical-basal axis; however, the molecular mechanisms accompanying this early event remain unclear. Here, we show that a small cysteine-rich peptide family is required for formation of the zygotic basal cell lineage and proembryo patterning in Arabidopsis. EMBRYO SURROUNDING FACTOR 1 (ESF1) peptides accumulate before fertilization in central cell gametes and thereafter in embryo-surrounding endosperm cells. Biochemical and structural analyses revealed cleavage of ESF1 propeptides to form biologically active mature peptides. Further, these peptides act in a non - cell-autonomous manner and synergistically with the receptor-like kinase SHORT SUSPENSOR to promote suspensor elongation through the YODA mitogen-activated protein kinase pathway. Our findings demonstrate that the second female gamete and its sexually derived endosperm regulate early embryonic patterning in flowering plants.
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UR - http://www.scopus.com/inward/citedby.url?scp=84897992428&partnerID=8YFLogxK
U2 - 10.1126/science.1243005
DO - 10.1126/science.1243005
M3 - Article
C2 - 24723605
AN - SCOPUS:84897992428
SN - 0036-8075
VL - 344
SP - 168
EP - 172
JO - Science
JF - Science
IS - 6180
ER -