TY - JOUR
T1 - Cerebellar regional dissection for molecular analysis
AU - Hamel, Katherine A.
AU - Cvetanovic, Marija
N1 - Publisher Copyright:
© 2020 JoVE Journal of Visualized Experiments.
PY - 2020/12
Y1 - 2020/12
N2 - Cerebellum plays an important role in several key functions including control of movement, balance, cognition, reward, and affect. Imaging studies indicate that distinct cerebellar regions contribute to these different functions. Molecular studies examining regional cerebellar differences are lagging as they are mostly done on whole cerebellar extracts thereby masking any distinctions across specific cerebellar regions. Here we describe a technique to reproducibly and quickly dissect four different cerebellar regions: the deep cerebellar nuclei (DCN), anterior and posterior vermal cerebellar cortex, and the cerebellar cortex of the hemispheres. Dissecting out these distinct regions allows for the exploration of molecular mechanisms that may underlie their unique contributions to balance, movement, affect and cognition. This technique may also be used to explore differences in pathological susceptibility of these specific regions across various mouse disease models.
AB - Cerebellum plays an important role in several key functions including control of movement, balance, cognition, reward, and affect. Imaging studies indicate that distinct cerebellar regions contribute to these different functions. Molecular studies examining regional cerebellar differences are lagging as they are mostly done on whole cerebellar extracts thereby masking any distinctions across specific cerebellar regions. Here we describe a technique to reproducibly and quickly dissect four different cerebellar regions: the deep cerebellar nuclei (DCN), anterior and posterior vermal cerebellar cortex, and the cerebellar cortex of the hemispheres. Dissecting out these distinct regions allows for the exploration of molecular mechanisms that may underlie their unique contributions to balance, movement, affect and cognition. This technique may also be used to explore differences in pathological susceptibility of these specific regions across various mouse disease models.
UR - http://www.scopus.com/inward/record.url?scp=85097778252&partnerID=8YFLogxK
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U2 - 10.3791/61922
DO - 10.3791/61922
M3 - Article
C2 - 33346205
AN - SCOPUS:85097778252
SN - 1940-087X
VL - 2020
SP - 1
EP - 13
JO - Journal of Visualized Experiments
JF - Journal of Visualized Experiments
IS - 166
M1 - e61922
ER -