cGMP-compliant expansion of human iPSC cultures as adherent monolayers

Ann M. Parr, Patrick J. Walsh, Vincent Truong, James R. Dutton

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations

Abstract

Therapeutic uses of cells differentiated from human pluripotent stem cells (hPSCs), either embryonic stem (ES) cells or induced pluripotent stem cells (iPSCs), are now being tested in clinical trials, and it is likely that this will lead to increased commercial interest in the clinical translation of promising hPSC research. Recent technical advances in the use of defined media and culture substrates have significantly improved both the simplicity and predictability of growing hPSCs, allowing a much more straightforward application of current good manufacturing practices (cGMP) to the culture of these cells. In addition, the adoption of cGMP-compliant techniques in research environments will both improve the replication of results and make the transition of promising investigations to the commercial sector significantly less cumbersome. However, passaging methods for hPSCs are inherently unpredictable and rely on operator experience and expertise. This is problematic for the cell manufacturing process where operator time and process predictability are often determining cost drivers.We have adopted a human iPSC system using defined media and a recombinant substrate that employs cell dissociation with a hypertonic citrate solution which eliminates variability during hPSC cell expansion and provides a simple cGMP-compliant technique for hiPSC cultivation that is appropriate in both research and commercial applications.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages221-229
Number of pages9
DOIs
StatePublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
Volume1357
ISSN (Print)1064-3745

Bibliographical note

Funding Information:
The authors acknowledge support from NIH grant U01HL100407 and the University of Minnesota Foundation.

Publisher Copyright:
© Springer Science+Business Media New York 2014.

Keywords

  • Cell manufacturing
  • Pluripotent stem cells
  • iPS cell culture

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