Changes in cell cycle-associated gene expression in a model of impaired liver regeneration

Jeffrey H Albrecht, Jeffrey S. Hoffman, Betsy T. Kren, Clifford J Steer

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Following partial hepatectomy (PH) there is compensatory regeneration of the remnant liver which eventually restores hepatic mass and function. The response to PH was studied in normal BALB/c and athymic nude mice, a model of impaired liver regeneration. Following PH, nude mice demonstrated diminished peak hepatic [3H]thymidine uptake and delayed liver mass restoration through 60 h post-PH. However, between 72-120 h there was no significant difference in mass restoration between the groups. The expression of genes associated with different stages of the cell cycle was evaluated in both models. In nude mice, there was an increase in peak expression of c-jun transcripts, while c-myc transcript expression was moderately attenuated. Thymidine kinase (TK) and cyclin-dependent kinase 1 (CDK1) mRNA expression was also diminished in athymic nude mice. The results suggest that while the defect in the regenerative response of the nude mouse after PH affects events in several phases of the cell cycle, mass restoration of the liver is only delayed and not attenuated.

Original languageEnglish (US)
Pages (from-to)157-162
Number of pages6
JournalFEBS Letters
Volume347
Issue number2-3
DOIs
StatePublished - Jun 27 1994

Bibliographical note

Funding Information:
AcknowledgemenTthsi:s studyw ass upportedin part by NationalI nsti-tutes of Health Grant ROl DK44649 to C.J.S. and a SmithKline BeechamP harmaceuticaltsr ainingg rantt o J.S.H.

Keywords

  • Athymic nude mouse
  • Cell cycle
  • Gene expression
  • Liver regeneration
  • Proto-oncogene
  • Transcript

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