Characteristics of Subpatent Malaria in a pre-elimination setting in Southern Zambia

Tamaki Kobayashi, Mufaro Kanyangarara, Natasha M. Laban, Masiliso Phiri, Harry Hamapumbu, Kelly M. Searle, Jennifer C. Stevenson, Philip E. Thuma, William J. Moss

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

To achieve and sustain malaria elimination, identification and treatment of the asymptomatic infectious reservoir is critical. Malaria rapid diagnostic tests (RDTs) are frequently used to identify asymptomatic, Plasmodiuminfected individuals through test-and-treat strategies, but their sensitivity is low when used in low transmission settings. Characteristics of individuals with subpatent (RDT-negative but polymerase chain reaction [PCR]-positive) Plasmodium parasitemia were evaluated in southern Zambia where malaria transmission has declined and efforts to achieve malaria elimination are underway. Simple random sampling based on satellite imagery was used to select households for participation in community-based, cross-sectional surveys between 2008 and 2013. Questionnaires were administered to collect information on age, gender, recent history of malaria symptoms, and recent antimalarial drug use. Blood samples were collected by finger prick for Plasmodium falciparum histidine-rich protein 2 RDT, blood smears for microscopy, and dried blood spots for molecular analysis to detect malaria parasites and their sexual stage. Of 3,863 participants with complete data, 102 (2.6%) were positive by microscopy, RDT, or PCR. Of these, 48 (47%) had subpatent parasitemia. Most individuals with subpatent parasitemia were asymptomatic (85%). Compared with individuals without parasitemia, individuals with subpatent parasitemia were significantly more likely to be aged 5-25 years. Approximately one quarter (27%) of those with subpatent parasitemia had detectable gametocytemia. These findings suggest that strategies based on active or reactive case detection can identify asymptomatic individuals positive by RDT, but more sensitive diagnostic tests or focal drug administration may be necessary to target individuals with subpatent parasitemia to achieve malaria elimination.

Original languageEnglish (US)
Pages (from-to)280-286
Number of pages7
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume100
Issue number2
DOIs
StatePublished - 2019
Externally publishedYes

Bibliographical note

Funding Information:
Acknowledgments: This work was supported by the Johns Hopkins Malaria Research Institute, Bloomberg Philanthropiesthe, and the Division of Microbiology and Infectious Diseases, National Institutes of Allergies and Infectious Diseases, National Institutes of Health as part of the International Centers of Excellence for Malaria Research (U19 AI089680). We thank the field team and laboratory staff at the Macha Research Trust and, most importantly, residents of the Macha community who participated in this study.

Publisher Copyright:
© 2019 by The American Society of Tropical Medicine and Hygiene.

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