Characterization, bioavailability and protective effects of phenolic-rich extracts from almond hulls against pro-oxidant induced toxicity in Caco-2 cells

Jun An; Juer Liu; Yuying Liang; Yiwei Ma; Chi Chen; Yanling Cheng; Peng Peng; Nan Zhou; Renchuan Zhang; Min Addy; Paul Chen; Yuhuan Liu; Guangwei Huang; Difeng Ren; Roger Ruan

doi:10.1016/j.foodchem.2020.126742

Characterization, bioavailability and protective effects of phenolic-rich extracts from almond hulls against pro-oxidant induced toxicity in Caco-2 cells

Jun An, Juer Liu, Yuying Liang, Yiwei Ma, Chi Chen, Yanling Cheng, Peng Peng, Nan Zhou, Renchuan Zhang, Min Addy, Paul Chen, Yuhuan Liu, Guangwei Huang, Difeng Ren, Roger Ruan

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21 Scopus citations

Abstract

Almond hulls, the main by-product of almond production, are considered a valuable source of bioactive phenolic compounds. This study aimed to characterize the phenolic composition, bioavailability of the phenolic-rich extracts from almond hulls (PEAH), and their protective effect on oxidative stressed Caco-2 cells induced by tert-butylhydroperoxide (t-BOOH). The ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS) analysis detected 11 phenolic compounds in the PEAH with high total phenolic content and antioxidant activity. Oxidative Caco-2 cell damage was reduced by PEAH, especially at 5 μg/mL, through scavenging reactive oxygen species (ROS), modulating the cellular endogenous antioxidant system and cell redox at a predictable status. Also, in vitro digestion influenced the phenolic compounds’ composition and antioxidant power of PEAH. These results suggested that almond hulls, rich in phenolic compounds, can meliorate the oxidative stressed Caco-2 cells and restore its impaired redox balance, and ultimately improve health benefits.

Original language	English (US)
Article number	126742
Journal	Food Chemistry
Volume	322
DOIs	https://doi.org/10.1016/j.foodchem.2020.126742
State	Published - Aug 30 2020

Bibliographical note

Funding Information:
This work was supported by grants from Almond Board of California , the China Scholarship Council Foundation (Grant Number 201806510024 ), the Fundamental Research Funds for the Central Universities (No. 2018BLRD08 ) and University of Minnesota Center for Biorefining.

Publisher Copyright:
© 2020 Elsevier Ltd

Keywords

Almond hulls
Antioxidant activity
Bioavailability
Characterization
Phenolic-rich extracts

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An, J., Liu, J., Liang, Y., Ma, Y., Chen, C., Cheng, Y., Peng, P., Zhou, N., Zhang, R., Addy, M., Chen, P., Liu, Y., Huang, G., Ren, D., & Ruan, R. (2020). Characterization, bioavailability and protective effects of phenolic-rich extracts from almond hulls against pro-oxidant induced toxicity in Caco-2 cells. Food Chemistry, 322, Article 126742. https://doi.org/10.1016/j.foodchem.2020.126742

An, J, Liu, J, Liang, Y, Ma, Y, Chen, C , Cheng, Y, Peng, P, Zhou, N, Zhang, R, Addy, M , Chen, P, Liu, Y, Huang, G, Ren, D & Ruan, R 2020, 'Characterization, bioavailability and protective effects of phenolic-rich extracts from almond hulls against pro-oxidant induced toxicity in Caco-2 cells', Food Chemistry, vol. 322, 126742. https://doi.org/10.1016/j.foodchem.2020.126742

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abstract = "Almond hulls, the main by-product of almond production, are considered a valuable source of bioactive phenolic compounds. This study aimed to characterize the phenolic composition, bioavailability of the phenolic-rich extracts from almond hulls (PEAH), and their protective effect on oxidative stressed Caco-2 cells induced by tert-butylhydroperoxide (t-BOOH). The ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS) analysis detected 11 phenolic compounds in the PEAH with high total phenolic content and antioxidant activity. Oxidative Caco-2 cell damage was reduced by PEAH, especially at 5 μg/mL, through scavenging reactive oxygen species (ROS), modulating the cellular endogenous antioxidant system and cell redox at a predictable status. Also, in vitro digestion influenced the phenolic compounds{\textquoteright} composition and antioxidant power of PEAH. These results suggested that almond hulls, rich in phenolic compounds, can meliorate the oxidative stressed Caco-2 cells and restore its impaired redox balance, and ultimately improve health benefits.",

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AU - Chen, Chi

AU - Cheng, Yanling

AU - Peng, Peng

AU - Zhou, Nan

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AU - Liu, Yuhuan

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Y1 - 2020/8/30

N2 - Almond hulls, the main by-product of almond production, are considered a valuable source of bioactive phenolic compounds. This study aimed to characterize the phenolic composition, bioavailability of the phenolic-rich extracts from almond hulls (PEAH), and their protective effect on oxidative stressed Caco-2 cells induced by tert-butylhydroperoxide (t-BOOH). The ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS) analysis detected 11 phenolic compounds in the PEAH with high total phenolic content and antioxidant activity. Oxidative Caco-2 cell damage was reduced by PEAH, especially at 5 μg/mL, through scavenging reactive oxygen species (ROS), modulating the cellular endogenous antioxidant system and cell redox at a predictable status. Also, in vitro digestion influenced the phenolic compounds’ composition and antioxidant power of PEAH. These results suggested that almond hulls, rich in phenolic compounds, can meliorate the oxidative stressed Caco-2 cells and restore its impaired redox balance, and ultimately improve health benefits.

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Characterization, bioavailability and protective effects of phenolic-rich extracts from almond hulls against pro-oxidant induced toxicity in Caco-2 cells

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Keywords

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