Characterization of α/β- and γ-gliadins in commercial varieties and breeding lines of durum wheat using MALDI-TOF and A-PAGE gels

Santiago Marín, Javier Gil-Humanes, Alberto Hernando, Francisco Barro

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4 Scopus citations

Abstract

In this work, gliadin composition has been analyzed in 33 accessions of durum wheat using MALDI-TOF MS and compared with A-PAGE results. The MALDI-TOF MS spectra were 29,900-42,500 Da, which corresponds to the α/β- and γ-gliadin regions in A-PAGE. The average of gliadin peaks per line was 23 for MALDI-TOF MS and only 14.8 bands for A-PAGE. MALDI-TOF MS identified 33 gliadin peaks in the durum wheat collection, 20 of which were unique peaks present in 7 lines. A-PAGE analysis identified 30 bands, of which only 4 were unique. Thus, the MALDI-TOF MS method was more sensitive than A-PAGE for identifying α/β- and γ-gliadins in the 33 durum wheat lines studied. Phylogenetic analyses performed using MALDI-TOF MS data assigned the durum wheat lines to two groups. The utility of MALDI-TOF MS to determine relationships among genotypes and for identification of durum wheat accessions is discussed.

Original languageEnglish (US)
Pages (from-to)735-747
Number of pages13
JournalBiochemical Genetics
Volume49
Issue number11-12
DOIs
StatePublished - Dec 2011

Bibliographical note

Funding Information:
Fig. 5 Mass spectra obtained from lines ‘T24’ and ‘Resende.’ ‘Resende’ is the line with the lowest number of gliadin peaks, and line ‘T24’ lacks the cluster of peaks in the α/β-gliadin region present in the other durum wheat lines. Molecular masses of the most persistent gliadin peaks are indicated in both lines. The unique peaks present in line ‘T24’ are marked by an asterisk (*) Acknowledgments This study was supported by the Spanish Comision Interministerial de Ciencia y Tecnología (AGL2010-19643-C02-02 and TRA2009_0047), the European Regional Development Fund (FEDER), and Junta de Andalucía (Project P09-AGR-4783). JG-H acknowledges financial support from the I3P Program of the Consejo Superior de Investigaciones Científicas, which is cofinanced by the European Social Fund. Technical assistance of Ana García is acknowledged. The authors thank Dr. Paul A. Lazzeri (Agrasys, S.L., 08028, Barcelona, Spain) for critical reading of the manuscript.

Keywords

  • Cereal breeding
  • Durum wheat
  • Gliadins
  • Mass spectrometry

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