Characterization of 17 polymorphic microsatellite loci in the Anise swallowtail, Papilio zelicaon (Lepidoptera: Papilionidae), and their amplification in related species

Evgueni V. Zakharov, Jessica J. Hellmann

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Fifteen polymorphic dinucleotide and two trinucleotide microsatellite loci were identified in the Anise swallowtail, Papilio zelicaon, from DNA genomic libraries enriched for simple sequence repeats. Allele numbers varied from eight to 29, with an excess of homozygotes observed for nine loci. This homozygosity is a feature of other lepidopteran microsatellites and is probably due to null alleles. Sixteen markers were amplified successfully in other representatives of Papilio with 11 loci retaining polymorphism in at least one species. These results suggest that the microsatellites reported here may be appropriate for measuring population genetic structure in a number of Papilio species.

Original languageEnglish (US)
Pages (from-to)144-146
Number of pages3
JournalMolecular Ecology Notes
Volume7
Issue number1
DOIs
StatePublished - Jan 2007
Externally publishedYes

Keywords

  • Codominant marker
  • Microsatellite DNA
  • Papilio
  • Simple sequence repeat
  • Swallowtail butterflies

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