Characterization of a cardiac complementary deoxyribonucleic acid library from the Turkey (Meleagris gallopavo)

K. M. Mendoza, W. Chiang, G. M. Strasburg, K. M. Reed

Research output: Contribution to journalArticlepeer-review

Abstract

Although the domestic turkey (Meleagris gallopavo) is a valuable agricultural commodity, genetic studies on this species lag behind those of other agricultural species. In this study, we examined expressed sequence tags (EST) from a turkey cardiac cDNA library constructed from 4 birds representing 2 developmental stages. A collection of 3,937 EST sequences were sequenced and analyzed for gene annotation and sequence variation. Clustering of sequences resulted in 353 contigs and 874 singletons (1,227 putative transcripts). All EST sequences were compared by BLASTN to the chicken whole genome sequence and to Ensembl and National Center for Biotechnology Information databases. The majority of significant matches correspond to genes found in the chicken. Sequence polymorphisms were identified in 310 contigs, 64 where the minor allele was observed to be present in more than 1 sequence. This study gives species-specific insight into the cardiac transcriptome of turkeys and provides resources for future studies of cardiac function.

Original languageEnglish (US)
Pages (from-to)1165-1170
Number of pages6
JournalPoultry science
Volume87
Issue number6
DOIs
StatePublished - Jun 1 2008

Bibliographical note

Funding Information:
This research was supported by a grant from the Cooperative State Research, Education and Extension Service, USDA (2005–35604–15628).

Keywords

  • Cardiac muscle
  • Cardiomyopathy
  • Expressed sequence tag
  • Round heart
  • Turkey

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