TY - JOUR
T1 - Characterization of a new, potent, immunopathogenic epitope in S-antigen that elicits T cells expressing Vβ8 and Vα2-like genes
AU - Merryman, Carmen F.
AU - Donoso, Larry A.
AU - Zhang, Xiangming
AU - Heber-Katz, Ellen
AU - Gregerson, Dale S.
PY - 1991/1/1
Y1 - 1991/1/1
N2 - Experimental autoimmune uveoretinitis (EAU) is a predominantly T cell-mediated autoimmune disease induced in susceptible animals by active immunization with human or bovine retinal S-Ag or by passive transfer of activated S-Ag or peptide-specific CD4+ T cells. During the course of studies aimed at the identification of T cell and B cell recognition sites in bovine and human S-Ag, a new potent uveitogenic region, located near the carboxy terminus of the molecule, was identified and characterized. Analysis of several synthetic peptides from this region showed that a 14 amino acid residue peptide, BSAg339-352, was highly uveitogenic when injected with adjuvants into Lewis rats. A uveitogenic T cell line, R737, was raised by in vitro selection of lymphocytes from animals immunized with peptide BSAg333-352. Northern blot analysis of mRNA from the R737 T cell line was positive for the rat homologs of murine Vβ8 and Vα2 T cell receptor gene probes. Whereas peptide BSAg339-352 defined the pathogenic site, nonpathogenic, proliferative sites were found in close physical association. This region is immediately adjacent to previously characterized pathogenic and proliferative sites contained in residues BSAg352-364. These results, as well as our previous observations, show S-Ag to be a complex molecule with several highly conserved amino acid sequences that can elicit pathogenic T cells with restricted T cell receptor gene usage capable of active and passive elicitation of experimental autoimmune uveoretinitis.
AB - Experimental autoimmune uveoretinitis (EAU) is a predominantly T cell-mediated autoimmune disease induced in susceptible animals by active immunization with human or bovine retinal S-Ag or by passive transfer of activated S-Ag or peptide-specific CD4+ T cells. During the course of studies aimed at the identification of T cell and B cell recognition sites in bovine and human S-Ag, a new potent uveitogenic region, located near the carboxy terminus of the molecule, was identified and characterized. Analysis of several synthetic peptides from this region showed that a 14 amino acid residue peptide, BSAg339-352, was highly uveitogenic when injected with adjuvants into Lewis rats. A uveitogenic T cell line, R737, was raised by in vitro selection of lymphocytes from animals immunized with peptide BSAg333-352. Northern blot analysis of mRNA from the R737 T cell line was positive for the rat homologs of murine Vβ8 and Vα2 T cell receptor gene probes. Whereas peptide BSAg339-352 defined the pathogenic site, nonpathogenic, proliferative sites were found in close physical association. This region is immediately adjacent to previously characterized pathogenic and proliferative sites contained in residues BSAg352-364. These results, as well as our previous observations, show S-Ag to be a complex molecule with several highly conserved amino acid sequences that can elicit pathogenic T cells with restricted T cell receptor gene usage capable of active and passive elicitation of experimental autoimmune uveoretinitis.
UR - http://www.scopus.com/inward/record.url?scp=0026019808&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026019808&partnerID=8YFLogxK
M3 - Article
C2 - 1701801
AN - SCOPUS:0026019808
SN - 0022-1767
VL - 146
SP - 75
EP - 80
JO - Journal of Immunology
JF - Journal of Immunology
IS - 1
ER -