TY - JOUR
T1 - Characterization of a novel S-RNase allele and genotyping of new apple cultivars
AU - Sheick, Ryan
AU - Serra, Sara
AU - Tillman, John
AU - Luby, James
AU - Evans, Kate
AU - Musacchi, Stefano
PY - 2020/11/17
Y1 - 2020/11/17
N2 - Apples (Malus × domestica Borkh.) are an economically important crop in many temperate growing regions around the world. Apple being characterized by gametophytic self-incompatibility (GSI) requires cross-pollination with compatible apple pollen during bloom to achieve sufficient fruit set for commercial production. For this reason, in commercial orchards it is common practice to plant pollinizer trees, which can be either crabapples or different apple cultivars, at a density of 5–10%. Crabapple trees, in addition to their ornamental traits, are valued as a source of genetic diversity for pollinating apple orchards. The genetics underlying cross-compatible responses among crabapples and domesticated apples, especially recently released cultivars, have been largely understudied. In this study, we characterized one novel S-RNase allele from the crabapple Malus ‘Doubloons’, named S59, and report an allele-specific PCR method for detection of this allele from genomic DNA. Further, we characterized the self-incompatibility genotypes (S-genotypes) of over twenty previously unreported Malus accessions, including some recent releases from the breeding programs of the University of Minnesota and Washington State University. The results of this work aim to provide new information about cross-compatibility of cultivars and pollinizers and may be used to aid parent selection in apple breeding programs, as well as pollinizer selection for the commercial orchard.
AB - Apples (Malus × domestica Borkh.) are an economically important crop in many temperate growing regions around the world. Apple being characterized by gametophytic self-incompatibility (GSI) requires cross-pollination with compatible apple pollen during bloom to achieve sufficient fruit set for commercial production. For this reason, in commercial orchards it is common practice to plant pollinizer trees, which can be either crabapples or different apple cultivars, at a density of 5–10%. Crabapple trees, in addition to their ornamental traits, are valued as a source of genetic diversity for pollinating apple orchards. The genetics underlying cross-compatible responses among crabapples and domesticated apples, especially recently released cultivars, have been largely understudied. In this study, we characterized one novel S-RNase allele from the crabapple Malus ‘Doubloons’, named S59, and report an allele-specific PCR method for detection of this allele from genomic DNA. Further, we characterized the self-incompatibility genotypes (S-genotypes) of over twenty previously unreported Malus accessions, including some recent releases from the breeding programs of the University of Minnesota and Washington State University. The results of this work aim to provide new information about cross-compatibility of cultivars and pollinizers and may be used to aid parent selection in apple breeding programs, as well as pollinizer selection for the commercial orchard.
KW - Apple
KW - cDNA
KW - Crabapple
KW - Genomic DNA sequences
KW - PCR detection
KW - S-alleles
UR - http://www.scopus.com/inward/record.url?scp=85088963095&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85088963095&partnerID=8YFLogxK
U2 - 10.1016/j.scienta.2020.109630
DO - 10.1016/j.scienta.2020.109630
M3 - Article
AN - SCOPUS:85088963095
VL - 273
JO - Scientia Horticulturae
JF - Scientia Horticulturae
SN - 0304-4238
M1 - 109630
ER -