TY - GEN
T1 - Characterization of cell association and heat treatment using iron oxide magnetic nanoparticles
AU - Kalambur, Venkat S.
AU - Longmire, Ellen
AU - Bischof, John C.
PY - 2007/12/1
Y1 - 2007/12/1
N2 - Magnetic iron oxide nanoparticles (NPs) have intrinsic advantages over other NPs for various biomedical applications. These advantages include visualization under Magnetic Resonance Imaging (MRI), heating with Radiofrequency (RF), and movement in a magnetic field. There are now numerous efforts to expand the applications of these particles for non-invasive drug and adjuvant delivery, cellular imaging and in vitro cell sorting and purification. In the present study, we describe methods to (i) assess and quantify NP cell association (ii) facilitate NP heat destruction of cells after association with RF and laser. First, we show that (i) the cell association of iron oxide NPs is dependent on the surface coating (surfactant greater than dextran), time, cell-type and extracellular NP concentrations (saturation with concentration and time). Furthermore, the association fits a simple enzyme Michealis-Menten model. Second, (ii) improved heat destruction of cells can be achieved after laser irradiation compared to traditional RF treatment for similar NP associations. These results and assays show promise for cell sorting and purification applications.
AB - Magnetic iron oxide nanoparticles (NPs) have intrinsic advantages over other NPs for various biomedical applications. These advantages include visualization under Magnetic Resonance Imaging (MRI), heating with Radiofrequency (RF), and movement in a magnetic field. There are now numerous efforts to expand the applications of these particles for non-invasive drug and adjuvant delivery, cellular imaging and in vitro cell sorting and purification. In the present study, we describe methods to (i) assess and quantify NP cell association (ii) facilitate NP heat destruction of cells after association with RF and laser. First, we show that (i) the cell association of iron oxide NPs is dependent on the surface coating (surfactant greater than dextran), time, cell-type and extracellular NP concentrations (saturation with concentration and time). Furthermore, the association fits a simple enzyme Michealis-Menten model. Second, (ii) improved heat destruction of cells can be achieved after laser irradiation compared to traditional RF treatment for similar NP associations. These results and assays show promise for cell sorting and purification applications.
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M3 - Conference contribution
AN - SCOPUS:40449098715
SN - 0791847985
SN - 9780791847985
T3 - Proceedings of the ASME Summer Bioengineering Conference 2007, SBC 2007
SP - 1053
EP - 1054
BT - Proceedings of the ASME Summer Bioengineering Conference 2007, SBC 2007
T2 - 2007 ASME Summer Bioengineering Conference, SBC 2007
Y2 - 20 June 2007 through 24 June 2007
ER -