Characterization of Salmonella enterica serovar Typhimurium DT104 invasion in an epithelial cell line (IPEC J2) from porcine small intestine

David R. Brown; Lisa D. Price

doi:10.1016/j.vetmic.2006.11.001

Characterization of Salmonella enterica serovar Typhimurium DT104 invasion in an epithelial cell line (IPEC J2) from porcine small intestine

David R. Brown, Lisa D. Price

Veterinary and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Salmonella Typhimurium DT104 is an emerging enteric pathogen in swine of increasing medical importance. In this study, the time course and the actin-dependent host signaling processes necessary for invasion of a S. Typhimurium DT104 field isolate were investigated in IPEC J2 epithelial cells derived from porcine small intestine. Internalized bacteria were quantified by a gentamicin resistance assay. DT104 internalization into epithelial monolayers increased steadily between 15 and 120 min after apical inoculation. Internalization was reduced by the Rho GTPase inhibitor mevastatin, the N-WASP inhibitor wiskostatin and the actin-disrupting agent cytochalasin D, but not the Rac1 GTPase inhibitor NSC-23766. Early DT104 invasion of porcine enterocytes appears to be mediated by Rac1 GTPase-independent changes in epithelial actin assembly.

Original language	English (US)
Pages (from-to)	328-333
Number of pages	6
Journal	Veterinary Microbiology
Volume	120
Issue number	3-4
DOIs	https://doi.org/10.1016/j.vetmic.2006.11.001
State	Published - Mar 10 2007

Bibliographical note

Funding Information:
This work was supported in part by National Institutes of Health grant R01 DA-10200.

Keywords

Actin
Intestinal epithelium
Neural Wiskott-Aldrich syndrome protein
Rho GTPase
Time course

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title = "Characterization of Salmonella enterica serovar Typhimurium DT104 invasion in an epithelial cell line (IPEC J2) from porcine small intestine",

abstract = "Salmonella Typhimurium DT104 is an emerging enteric pathogen in swine of increasing medical importance. In this study, the time course and the actin-dependent host signaling processes necessary for invasion of a S. Typhimurium DT104 field isolate were investigated in IPEC J2 epithelial cells derived from porcine small intestine. Internalized bacteria were quantified by a gentamicin resistance assay. DT104 internalization into epithelial monolayers increased steadily between 15 and 120 min after apical inoculation. Internalization was reduced by the Rho GTPase inhibitor mevastatin, the N-WASP inhibitor wiskostatin and the actin-disrupting agent cytochalasin D, but not the Rac1 GTPase inhibitor NSC-23766. Early DT104 invasion of porcine enterocytes appears to be mediated by Rac1 GTPase-independent changes in epithelial actin assembly.",

keywords = "Actin, Intestinal epithelium, Neural Wiskott-Aldrich syndrome protein, Rho GTPase, Time course",

author = "Brown, {David R.} and Price, {Lisa D.}",

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doi = "10.1016/j.vetmic.2006.11.001",

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AU - Brown, David R.

AU - Price, Lisa D.

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AB - Salmonella Typhimurium DT104 is an emerging enteric pathogen in swine of increasing medical importance. In this study, the time course and the actin-dependent host signaling processes necessary for invasion of a S. Typhimurium DT104 field isolate were investigated in IPEC J2 epithelial cells derived from porcine small intestine. Internalized bacteria were quantified by a gentamicin resistance assay. DT104 internalization into epithelial monolayers increased steadily between 15 and 120 min after apical inoculation. Internalization was reduced by the Rho GTPase inhibitor mevastatin, the N-WASP inhibitor wiskostatin and the actin-disrupting agent cytochalasin D, but not the Rac1 GTPase inhibitor NSC-23766. Early DT104 invasion of porcine enterocytes appears to be mediated by Rac1 GTPase-independent changes in epithelial actin assembly.

KW - Actin

KW - Intestinal epithelium

KW - Neural Wiskott-Aldrich syndrome protein

KW - Rho GTPase

KW - Time course

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Characterization of Salmonella enterica serovar Typhimurium DT104 invasion in an epithelial cell line (IPEC J2) from porcine small intestine

Abstract

Bibliographical note

Keywords

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