Chemical analysis of aquatic pheromones in fish

Michael Stewart, Cindy F. Baker, Peter W. Sorensen

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Pheromones are chemicals that pass between members of the same species that have inherent meaning. In the case of fish, pheromones are water-soluble and found in low concentrations. As such, sensitive and selective methods are needed to separate and analyze these pheromones from an environmental matrix that may contain many other chemicals. This chapter describes a generic method used to concentrate and identify these chemicals and two extremely sensitive and selective methods for analysis, namely, mass spectrometry and enzyme-linked immunosorbent assay.

Original languageEnglish (US)
Title of host publicationPheromone Signaling
Subtitle of host publicationMethods and Protocols
EditorsKazushige Touhara
Pages55-69
Number of pages15
DOIs
StatePublished - Sep 20 2013

Publication series

NameMethods in Molecular Biology
Volume1068
ISSN (Print)1064-3745

Keywords

  • ELISA
  • Enzyme-linked immunosorbent assay
  • Fish pheromone
  • Liquid chromatography
  • Mass spectrometry
  • Solid phase extraction chromatography

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