TY - JOUR
T1 - Chemically induced self-assembly of enzyme nanorings.
AU - White, Brian R.
AU - Li, Qing
AU - Wagner, Carston R.
PY - 2011
Y1 - 2011
N2 - Continued exploration into the field of chemically induced dimerization (CID) has revealed a number of applications for its use in a broader context as a method of structural assembly (1-4). In particular, the use of CID technology to generate self-assembled (and selectively disassembled) protein toroids serves as a key advancement toward developing stable and controllable protein-based platforms. Such structures have broad application to the development of novel therapeutics, lab-on-a-chip technologies, and multi-enzyme assemblies (5, 6). This chapter describes a method of developing an enzymatically active protein nanostructure incorporating both a CID-based assembly region containing dihydrofolate reductase (DHFR) and an enzymatic region consisting of histidine triad nucleotide binding protein 1 (Hint1). Details of both the production and the characterization of this structure are provided.
AB - Continued exploration into the field of chemically induced dimerization (CID) has revealed a number of applications for its use in a broader context as a method of structural assembly (1-4). In particular, the use of CID technology to generate self-assembled (and selectively disassembled) protein toroids serves as a key advancement toward developing stable and controllable protein-based platforms. Such structures have broad application to the development of novel therapeutics, lab-on-a-chip technologies, and multi-enzyme assemblies (5, 6). This chapter describes a method of developing an enzymatically active protein nanostructure incorporating both a CID-based assembly region containing dihydrofolate reductase (DHFR) and an enzymatic region consisting of histidine triad nucleotide binding protein 1 (Hint1). Details of both the production and the characterization of this structure are provided.
UR - http://www.scopus.com/inward/record.url?scp=80052617402&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80052617402&partnerID=8YFLogxK
U2 - 10.1007/978-1-61779-132-1_2
DO - 10.1007/978-1-61779-132-1_2
M3 - Article
C2 - 21553179
AN - SCOPUS:80052617402
SN - 1064-3745
VL - 743
SP - 17
EP - 26
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -