Chemically induced self-assembly of enzyme nanorings.

Brian R. White, Qing Li, Carston R. Wagner

Research output: Contribution to journalArticlepeer-review

Abstract

Continued exploration into the field of chemically induced dimerization (CID) has revealed a number of applications for its use in a broader context as a method of structural assembly (1-4). In particular, the use of CID technology to generate self-assembled (and selectively disassembled) protein toroids serves as a key advancement toward developing stable and controllable protein-based platforms. Such structures have broad application to the development of novel therapeutics, lab-on-a-chip technologies, and multi-enzyme assemblies (5, 6). This chapter describes a method of developing an enzymatically active protein nanostructure incorporating both a CID-based assembly region containing dihydrofolate reductase (DHFR) and an enzymatic region consisting of histidine triad nucleotide binding protein 1 (Hint1). Details of both the production and the characterization of this structure are provided.

Original languageEnglish (US)
Pages (from-to)17-26
Number of pages10
JournalMethods in molecular biology (Clifton, N.J.)
Volume743
DOIs
StatePublished - 2011

Fingerprint Dive into the research topics of 'Chemically induced self-assembly of enzyme nanorings.'. Together they form a unique fingerprint.

Cite this