TY - JOUR
T1 - Chromatographic and kinetic properties of acid- and pepsin-activated inactive renin from human amniotic fluid
AU - Johnson, Rodney L.
AU - Fleming, Neal W.
AU - Poisner, Alan M.
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1979/9/1
Y1 - 1979/9/1
N2 - The Chromatographic and kinetic properties of acid- and pepsin-activated inactive renin from human amniotic fluid were determined. Acid-activated inactive renin, like inactive renin, was found to be bound to an Affi-Gel Blue affinity column. Pepsin-activated inactive renin, on the other hand, did not bind to such a column. The Km values of endogenous active renin, acid-activated inactive renin, and pepsin-activated inactive renin with bovine substrate were 0.11, 0.12 and 0.05 μM respectively. With hog substrate, the Km values were 0.16,0.19 and 0.10 μM respectively. These results suggest that there is a difference between the active form of renin obtained from acid activation of inactive renin and the active renin obtained after pepsin treatment.
AB - The Chromatographic and kinetic properties of acid- and pepsin-activated inactive renin from human amniotic fluid were determined. Acid-activated inactive renin, like inactive renin, was found to be bound to an Affi-Gel Blue affinity column. Pepsin-activated inactive renin, on the other hand, did not bind to such a column. The Km values of endogenous active renin, acid-activated inactive renin, and pepsin-activated inactive renin with bovine substrate were 0.11, 0.12 and 0.05 μM respectively. With hog substrate, the Km values were 0.16,0.19 and 0.10 μM respectively. These results suggest that there is a difference between the active form of renin obtained from acid activation of inactive renin and the active renin obtained after pepsin treatment.
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U2 - 10.1016/0006-2952(79)90033-9
DO - 10.1016/0006-2952(79)90033-9
M3 - Article
C2 - 42402
AN - SCOPUS:0018666551
SN - 0006-2952
VL - 28
SP - 2597
EP - 2600
JO - Biochemical Pharmacology
JF - Biochemical Pharmacology
IS - 17
ER -