Chromosomal Localization of Angiotensin II Type 1 Receptor Isoforms in the Rat

J. L. Lewis, T. Serikawa, D. G. Warnock

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32 Scopus citations

Abstract

The cDNA sequences of two different isoforms of the rat angiotensin II type 1 receptors, AT1A and AT1B, have been reported. A single set of polymerase chain reaction primers was used to amplify sequence from both AT1A and AT1B from rat genomic DNA. Genomic DNA from a panel of rat × mouse somatic hybrid cell lines which had been characterized as to the rat chromosomal content was then amplified with these primers. The amplified products from rat AT1A and AT1B were distinguished from each other and those of the mouse by the use of differential restriction patterns. Using this method, AT1A was localized to rat chromosome 17 and AT1B to rat chromosome 2.

Original languageEnglish (US)
Pages (from-to)677-682
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume194
Issue number2
DOIs
StatePublished - Jul 30 1993

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