Objective: Biallelic ablation of VGF determines a dwarf phenotype. VGF precursor protein encodes for different biologically active peptides none of which has been related to growth or muscular abnormalities. Here we present the first attempt to fill this gap. We tested the hypothesis that a recently identified VGF-derived peptide, TLQP-21, shown to centrally modulate metabolic functions, could also modulate growth hormone (GH)-axis and muscle strength. Design: Adult male mice were chronically icv injected with TLQP-21 (15 μg/day for 14 days). Physiological, molecular and behavioral parameters related to the GH/IGF-1-axis were investigated. Results: Except for a reduction in the soleus weight, TLQP-21 did not affect GH/IGF-1-axis mediators, muscle strength and muscle weight. Conclusions: Results collected exclude a role for TLQP-21 in modulating the GH/IGF1-axis and muscle functions. VGF-derived peptides involved in the dwarf phenotype of VGF-/- mice have to be identified yet.
Bibliographical noteFunding Information:
Author wish to thanks Silvia Mandillo and Foteini Mourkioti for help with the grip strength test and muscle dissection, respectively. Supported by FIRB RBNE01JKLF_ 004 to AM; FIRB RBNE01JKLF_006, FIRB RBNE013XSJ_004 and IZS SA 002/02 to RP; FIRB RBNE01JKLF_008 to VL and Fondo di Ateneo per la Ricerca of the Univ. Milano-Bicocca to AT and VL; FIRB RBNE01JKLF_ 001 to EEM; FIRS Neurobiotecnologie and Telethon E0830 to AL.
- Grip strength test