As a prelude to assessing the relationship of chromosome alterations to clinical outcome in ovarian carcinoma, we report on the cytogenetic analysis on short-term cultures from 54 patients. All patients had histopathologically confirmed malignancy, with the majority of cases demonstrating serous ovarian adenocarcinomas. Structural alterations were evident in 52 cases, whereas numeric changes were identified in 13 cases. The most notable numeric abnormalities were loss of the X-chromosome ( 9 13 total cases) and +7 ( 3 9 diploid cases). Structural alterations most frequently involved chromosomes 1, 3, 6, 7, 11, and 12. Chromosomal breakpoints were shown to cluster in several chromosomal banding regions, including 1p36, 1p11-q21, 3p23-p10, 7p (especially 7p22), 11p, 11q, 12p13-q12, and 12q24. The frequency of structural alterations involving the following chromosome arms was found to be significantly increased: 1p (p < 0.01), 7p (p < 0.01), 11p (p < 0.01), 11q (p < 0.05), and 12p (p < 0.05). An analysis of the net gain or loss of chromosome segments was also performed, with the most consistent tendency observed being over-representation of 1q and chromosome 7, deletion of 1p, and loss of the X chromosome.
Bibliographical noteFunding Information:
We would like to acknowledge members of the Gynecologic Cancer Committee of the Southwest Oncology Group for forwarding fresh humAr~ ovarian cancers to the Arizona Cancer Center for cytogenctic analysis. The major tumor contributors include Drs. Earl Surwit, A1 Bonebrake, Darryl Wallace, Ken Hatch, and Francisco Ampuero. We would also like to thank Lee Wisner for technical assistance in the preparation of the figures. This work is supported by NCI grant CA-41183.