Cloning and expression of a catalytic core bovine brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase

Francese Ventura, Santi Ambrosio, Ramon Bartrons, M. Raafat El-Maghrabi, Alex J. Lange, Simon J. Pilkis

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

A cDNA encoding the catalytic core of a novel brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase isoenzyme was isolated from a λgt10 bovine brain library. This brain cDNA begins and ends in an open reading frame encoding a peptide of 476 amino acids. This peptide contains both the catalytic kinase and bisphosphatase domains and has an overall 65% and 67% identity with the bovine heart and liver isozymes, respectively, whereas the NH2 and COOH-termini are divergent. An active catalytic core brain bifunctional enzyme was expressed in E. coli using a T7 RNA polymerase-based expression system. These results support the presence of a distinct gene coding for the protein in bovine brain.

Original languageEnglish (US)
Pages (from-to)1140-1148
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume209
Issue number3
DOIs
StatePublished - Apr 26 1995

Bibliographical note

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Copyright 2017 Elsevier B.V., All rights reserved.

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