The molecular basis of bacteriocin production by a Cicer - Rhizobium strain PR2109a was studied. The bacterial strain showed in vitro growth inhibition of non-bacteriocin producing strain of Cicer - Rhizobium PR2005b. Tn5 mutagenesis of the wild-type strain helped in the isolation of the bacteriocin-defective mutant JN365. A genomic library of the wild-type strain was constructed in the cosmid vector pLAFR1 and maintained in Escherchia coli background. Complementation analysis with the cosmid library resulted in the isolation of a cosmid clone which complemented the defective character in the mutant JN365. The size of the complementary DNA fragment was found to be 23 kb.
Bibliographical noteFunding Information:
The author (J.N.) thanks the Indian Council of Agricultural Research, New Delhi for the senior research fellowship provided during the study.
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- Cicer - Rhizobium