Abstract
The molecular basis of bacteriocin production by a Cicer - Rhizobium strain PR2109a was studied. The bacterial strain showed in vitro growth inhibition of non-bacteriocin producing strain of Cicer - Rhizobium PR2005b. Tn5 mutagenesis of the wild-type strain helped in the isolation of the bacteriocin-defective mutant JN365. A genomic library of the wild-type strain was constructed in the cosmid vector pLAFR1 and maintained in Escherchia coli background. Complementation analysis with the cosmid library resulted in the isolation of a cosmid clone which complemented the defective character in the mutant JN365. The size of the complementary DNA fragment was found to be 23 kb.
| Original language | English (US) |
|---|---|
| Article number | 324847 |
| Pages (from-to) | 47-49 |
| Number of pages | 3 |
| Journal | World Journal of Microbiology and Biotechnology |
| Volume | 17 |
| Issue number | 1 |
| DOIs | |
| State | Published - 2001 |
Bibliographical note
Funding Information:The author (J.N.) thanks the Indian Council of Agricultural Research, New Delhi for the senior research fellowship provided during the study.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
Keywords
- Bacteriocin
- Cicer - Rhizobium
- Competitiveness