Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor

Linda S. Park; Unja Martin; Kirsten Garka; Brian Gliniak; James P. Di Santo; Werner Muller; David A. Largaespada; Neal G. Copeland; Nancy A. Jenkins; Andrew G. Farr; Steven F. Ziegler; Philip J. Morrissey; Raymond Paxton; John E. Sims

doi:10.1084/jem.192.5.659

Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor

Linda S. Park, Unja Martin, Kirsten Garka, Brian Gliniak, James P. Di Santo, Werner Muller, David A. Largaespada, Neal G. Copeland, Nancy A. Jenkins, Andrew G. Farr, Steven F. Ziegler, Philip J. Morrissey, Raymond Paxton, John E. Sims

Pediatrics - Hematology

Research output: Contribution to journal › Article › peer-review

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Abstract

The cellular receptor for murine thymic stromal lymphopoietin (TSLP) was detected in a variety of murine, but not human myelomonocytic cell lines by radioligand binding. cDNA clones encoding the receptor were isolated from a murine T helper cell cDNA library. TSLP receptor (TSLPR) is a member of the hematopoietin receptor family. Transfection of TSLPR cDNA resulted in only low affinity binding. Cotransfection of the interleukin 7 (IL-7)Rα chain cDNA resulted in conversion to high affinity binding. TSLP did not activate cells from IL-7Rα(-/-) mice, but did activate cells from γc(-/-) mice. Thus, the functional TSLPR requires the IL-7Rα chain, but not the γc chain for signaling.

Original language	English (US)
Pages (from-to)	659-669
Number of pages	11
Journal	Journal of Experimental Medicine
Volume	192
Issue number	5
DOIs	https://doi.org/10.1084/jem.192.5.659
State	Published - Sep 4 2000

Keywords

Cytokine
Cytokine receptors
DNA sequence
Interleukin 2 receptor
Interleukin 7 receptor

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Park, L. S., Martin, U., Garka, K., Gliniak, B., Di Santo, J. P., Muller, W., Largaespada, D. A., Copeland, N. G., Jenkins, N. A., Farr, A. G., Ziegler, S. F., Morrissey, P. J., Paxton, R., & Sims, J. E. (2000). Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor. Journal of Experimental Medicine, 192(5), 659-669. https://doi.org/10.1084/jem.192.5.659

Park, LS, Martin, U, Garka, K, Gliniak, B, Di Santo, JP, Muller, W, Largaespada, DA, Copeland, NG, Jenkins, NA, Farr, AG, Ziegler, SF, Morrissey, PJ, Paxton, R & Sims, JE 2000, 'Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor', Journal of Experimental Medicine, vol. 192, no. 5, pp. 659-669. https://doi.org/10.1084/jem.192.5.659

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abstract = "The cellular receptor for murine thymic stromal lymphopoietin (TSLP) was detected in a variety of murine, but not human myelomonocytic cell lines by radioligand binding. cDNA clones encoding the receptor were isolated from a murine T helper cell cDNA library. TSLP receptor (TSLPR) is a member of the hematopoietin receptor family. Transfection of TSLPR cDNA resulted in only low affinity binding. Cotransfection of the interleukin 7 (IL-7)Rα chain cDNA resulted in conversion to high affinity binding. TSLP did not activate cells from IL-7Rα(-/-) mice, but did activate cells from γc(-/-) mice. Thus, the functional TSLPR requires the IL-7Rα chain, but not the γc chain for signaling.",

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KW - DNA sequence

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Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor

Abstract

Keywords

UN SDGs

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