TY - GEN
T1 - Cloning, phylogenetic and expression analysis of soybean ferritin gene
AU - Lv, Shengjun
AU - Xu, Hongliang
AU - Wang, Pengchao
AU - Li, Wenhui
AU - Li, Yaxuan
AU - Hu, Yingkao
PY - 2011/9/5
Y1 - 2011/9/5
N2 - To better understand soybean ferritin gene structure and its expression, a full length of 753bp cDNA fragment, which encoded a 250 amino acids peptide, was cloned by RT-PCR from a cultivar of Zhong Huang 13. Multiple sequence alignment and phylogenetic analysis were performed with ferritins from 6 species including Glycine max-, Arabidopsis thaliana, Oryza sativa, Physcomitrella patens, Brachypodium distachyon and Chlamydomonas. The results showed that gene lost occurred during the evolution of ferritin, and paralogs were widespread. Gene structure and phylogenetic analysis revealed that this soybean ferritin gene located on chromosome 18 with 8 exons and 7 introns and had high identity to other legume genes. Promoter analysis showed that a lot of stress responsive elements existed in this region including core promoter elements, indicates its possible function in soybean stress response. In silico expression revealed by Unigene database from NCBI did not give an obviously organ-specific expression pattern, but the expression level in green organ was higher than in other.
AB - To better understand soybean ferritin gene structure and its expression, a full length of 753bp cDNA fragment, which encoded a 250 amino acids peptide, was cloned by RT-PCR from a cultivar of Zhong Huang 13. Multiple sequence alignment and phylogenetic analysis were performed with ferritins from 6 species including Glycine max-, Arabidopsis thaliana, Oryza sativa, Physcomitrella patens, Brachypodium distachyon and Chlamydomonas. The results showed that gene lost occurred during the evolution of ferritin, and paralogs were widespread. Gene structure and phylogenetic analysis revealed that this soybean ferritin gene located on chromosome 18 with 8 exons and 7 introns and had high identity to other legume genes. Promoter analysis showed that a lot of stress responsive elements existed in this region including core promoter elements, indicates its possible function in soybean stress response. In silico expression revealed by Unigene database from NCBI did not give an obviously organ-specific expression pattern, but the expression level in green organ was higher than in other.
KW - Ferritin
KW - Gene cloning
KW - In silico expression
KW - Promoter analysis
UR - http://www.scopus.com/inward/record.url?scp=80052219033&partnerID=8YFLogxK
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U2 - 10.1109/RSETE.2011.5966091
DO - 10.1109/RSETE.2011.5966091
M3 - Conference contribution
AN - SCOPUS:80052219033
SN - 9781424491711
T3 - 2011 International Conference on Remote Sensing, Environment and Transportation Engineering, RSETE 2011 - Proceedings
SP - 7446
EP - 7449
BT - 2011 International Conference on Remote Sensing, Environment and Transportation Engineering, RSETE 2011 - Proceedings
T2 - 2011 International Conference on Remote Sensing, Environment and Transportation Engineering, RSETE 2011
Y2 - 24 June 2011 through 26 June 2011
ER -