Cloning, sequencing and regulation of an mRNA encoding porcine interleukin-lβ

Michael J. Huether, Gaofeng Lin, Doug M. Smith, Michael P Murtaugh, Thomas W Molitor

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Interleukin 1 (IL-1) is a cytokine that mediates a variety of immunological responses. We report here the isolation of a porcine IL-1β cDNA from an alveolar macrophage library. The complete cDNA sequence is 1458 bp in length and has an open reading frame of 803 bp, encoding a 267-amino-acid (aa) protein with an approximate molecular size of 31 kDa. The porcine IL-1β cDNA sequence shows homologies of 82.0, 81.0, 74.0, and 74.0% with the bovine, sheep, human, and murine sequences in the protein coding regions, respectively. Northern blot analysis reveals a single 1.7-kb mRNA species that is differentially regulated by lipopolysaccharide, phorbol myristate acetate, and tumor necrosis factor but is not regulated by human recombinant IL-1. Porcine IL-1β is down-regulated by dexamethasone, polymyxin B, and IL-4 in a fashion kinetically similar to the other reported species.

Original languageEnglish (US)
Pages (from-to)285-289
Number of pages5
JournalGene
Volume129
Issue number2
DOIs
StatePublished - Jul 30 1993

Keywords

  • IL-Iβ
  • PCR
  • Recombinant DNA
  • comparison
  • cytokine
  • nucleotide sequence

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