Cloning, sequencing and regulation of an mRNA encoding porcine interleukin-lβ

Michael J. Huether; Gaofeng Lin; Doug M. Smith; Michael P Murtaugh; Thomas W Molitor

doi:10.1016/0378-1119(93)90281-7

Cloning, sequencing and regulation of an mRNA encoding porcine interleukin-lβ

Michael J. Huether, Gaofeng Lin, Doug M. Smith, Michael P Murtaugh, Thomas W Molitor

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Abstract

Interleukin 1 (IL-1) is a cytokine that mediates a variety of immunological responses. We report here the isolation of a porcine IL-1β cDNA from an alveolar macrophage library. The complete cDNA sequence is 1458 bp in length and has an open reading frame of 803 bp, encoding a 267-amino-acid (aa) protein with an approximate molecular size of 31 kDa. The porcine IL-1β cDNA sequence shows homologies of 82.0, 81.0, 74.0, and 74.0% with the bovine, sheep, human, and murine sequences in the protein coding regions, respectively. Northern blot analysis reveals a single 1.7-kb mRNA species that is differentially regulated by lipopolysaccharide, phorbol myristate acetate, and tumor necrosis factor but is not regulated by human recombinant IL-1. Porcine IL-1β is down-regulated by dexamethasone, polymyxin B, and IL-4 in a fashion kinetically similar to the other reported species.

Original language	English (US)
Pages (from-to)	285-289
Number of pages	5
Journal	Gene
Volume	129
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(93)90281-7
State	Published - Jul 30 1993

Keywords

IL-Iβ
PCR
Recombinant DNA
comparison
cytokine
nucleotide sequence

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title = "Cloning, sequencing and regulation of an mRNA encoding porcine interleukin-lβ",

abstract = "Interleukin 1 (IL-1) is a cytokine that mediates a variety of immunological responses. We report here the isolation of a porcine IL-1β cDNA from an alveolar macrophage library. The complete cDNA sequence is 1458 bp in length and has an open reading frame of 803 bp, encoding a 267-amino-acid (aa) protein with an approximate molecular size of 31 kDa. The porcine IL-1β cDNA sequence shows homologies of 82.0, 81.0, 74.0, and 74.0% with the bovine, sheep, human, and murine sequences in the protein coding regions, respectively. Northern blot analysis reveals a single 1.7-kb mRNA species that is differentially regulated by lipopolysaccharide, phorbol myristate acetate, and tumor necrosis factor but is not regulated by human recombinant IL-1. Porcine IL-1β is down-regulated by dexamethasone, polymyxin B, and IL-4 in a fashion kinetically similar to the other reported species.",

keywords = "IL-Iβ, PCR, Recombinant DNA, comparison, cytokine, nucleotide sequence",

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AU - Huether, Michael J.

AU - Lin, Gaofeng

AU - Smith, Doug M.

AU - Murtaugh, Michael P

AU - Molitor, Thomas W

PY - 1993/7/30

Y1 - 1993/7/30

N2 - Interleukin 1 (IL-1) is a cytokine that mediates a variety of immunological responses. We report here the isolation of a porcine IL-1β cDNA from an alveolar macrophage library. The complete cDNA sequence is 1458 bp in length and has an open reading frame of 803 bp, encoding a 267-amino-acid (aa) protein with an approximate molecular size of 31 kDa. The porcine IL-1β cDNA sequence shows homologies of 82.0, 81.0, 74.0, and 74.0% with the bovine, sheep, human, and murine sequences in the protein coding regions, respectively. Northern blot analysis reveals a single 1.7-kb mRNA species that is differentially regulated by lipopolysaccharide, phorbol myristate acetate, and tumor necrosis factor but is not regulated by human recombinant IL-1. Porcine IL-1β is down-regulated by dexamethasone, polymyxin B, and IL-4 in a fashion kinetically similar to the other reported species.

AB - Interleukin 1 (IL-1) is a cytokine that mediates a variety of immunological responses. We report here the isolation of a porcine IL-1β cDNA from an alveolar macrophage library. The complete cDNA sequence is 1458 bp in length and has an open reading frame of 803 bp, encoding a 267-amino-acid (aa) protein with an approximate molecular size of 31 kDa. The porcine IL-1β cDNA sequence shows homologies of 82.0, 81.0, 74.0, and 74.0% with the bovine, sheep, human, and murine sequences in the protein coding regions, respectively. Northern blot analysis reveals a single 1.7-kb mRNA species that is differentially regulated by lipopolysaccharide, phorbol myristate acetate, and tumor necrosis factor but is not regulated by human recombinant IL-1. Porcine IL-1β is down-regulated by dexamethasone, polymyxin B, and IL-4 in a fashion kinetically similar to the other reported species.

KW - IL-Iβ

KW - PCR

KW - Recombinant DNA

KW - comparison

KW - cytokine

KW - nucleotide sequence

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Cloning, sequencing and regulation of an mRNA encoding porcine interleukin-lβ

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Keywords

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