Coarse-grained dynamics of protein synthesis in a cell-free system

Eyal Karzbrun, Jonghyeon Shin, Roy H. Bar-Ziv, Vincent Noireaux

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83 Scopus citations

Abstract

A complete gene expression reaction is reconstituted in a cell-free system comprising the entire endogenous transcription, translation, as well as mRNA and protein degradation machinery of E. coli. In dissecting the major reaction steps, we derive a coarse-grained enzymatic description of biosynthesis and degradation, from which ten relevant rate constants and concentrations are determined. Governed by zeroth-order degradation, protein expression follows a sharp transition from undetectable levels to constant-rate accumulation, without reaching steady state.

Original languageEnglish (US)
Article number048104
JournalPhysical review letters
Volume106
Issue number4
DOIs
StatePublished - Jan 24 2011

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