Cold hardiness and deacclimation of overwintering Papilio zelicaon pupae

Caroline M. Williams, Annegret Nicolai, Laura V. Ferguson, Mark A. Bernards, Jessica J. Hellmann, Brent J. Sinclair

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Seasonally-acquired cold tolerance can be reversed at warm temperatures, leaving temperate ectotherms vulnerable to cold snaps. However, deacclimation, and its underlying mechanisms, has not been well-explored in insects. Swallowtail butterflies are widely distributed but in some cases their range is limited by low temperature and their cold tolerance is seasonally acquired, implying that they experience mortality resulting from deacclimation. We investigated cold tolerance and hemolymph composition of Anise swallowtail (Papilio zelicaon) pupae during overwintering in the laboratory, and after four days exposure to warm temperatures in spring. Overwintering pupae had supercooling points around -20.5°C and survived brief exposures to -30°C, suggesting partial freeze tolerance. Overwintering pupae had hemolymph osmolality of approximately 920mOsm, imparted by high concentrations of glycerol, K+ and Na+. After exposure to spring warming, supercooling points increased to approximately -17°C, and survival of a 1h exposure to -20°C decreased from 100% to 0%. This deacclimation was associated with decreased hemolymph osmolality and reduced glycerol, trehalose, Na+ and Ca2+ concentrations. We compared cold tolerance of pupae to weather conditions at and beyond the species' northern range boundary. Minimum temperatures at the range boundary approached the lower lethal temperature of pupae, and were colder north of the range, suggesting that cold hardiness may set northern range limits. Minimum temperatures following warm snaps were likely to cause mortality in at least one of the past three years. Cold snaps in the spring are increasing in frequency as a result of global climate change, so are likely to be a significant source of mortality for this species, and other temperate ectotherms.

Original languageEnglish (US)
Pages (from-to)51-58
Number of pages8
JournalComparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology
Volume178
DOIs
StatePublished - Dec 2014
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by an NSERC Discovery Grant , the Canadian Foundation for Innovation and Ontario Ministry for Research and Innovation Early Researcher award to BJS, a DOE grant ( DEFG-02-05ER ) to JJH, an NSERC Discovery Grant award to MAB and an Ontario Graduate Scholarship to CMW. CMW was supported by NSF grant 1051890 to Daniel A. Hahn during preparation of this manuscript. Thanks to two anonymous referees for constructive comments that improved an earlier version of the ms.

Keywords

  • Climate change
  • Cold tolerance
  • Cryoprotectant
  • Deacclimation
  • Hemolymph
  • Insect
  • Lepidoptera
  • Winter

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