The neonatal smooth muscle cells (SMC) produced more collagen when seeded in fibrin gel rather than collagen gel. The quantity of cross-linked elastic fibers was enhanced by treatment with TGFβ1 and insulin. The When compared to collagen ME , the fibrin ME compacted to a greater extent and were both stronger and stiffer when cultured under the same conditions, having a tensile modulus and ultimate tensile strength (UTS). It was found that the use of soluble factors increased elastin production, as measured by alkaline extraction and amino acid analysis, 5-fold in fibrin gels, which was about 4 times greater than in collagen gels.
|Original language||English (US)|
|Number of pages||1|
|State||Published - Dec 1 2002|
|Event||Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products - Atlanta, GA, United States|
Duration: Oct 13 2002 → Oct 16 2002
|Other||Third Smith and Nephew International Symposium - Translating Tissue Engineering into Products|
|Period||10/13/02 → 10/16/02|