Collinearity analysis and high-density genetic mapping of the wheat powdery mildew resistance gene Pm40 in PI 672538

Shengfu Zhong, Lixia Ma, Syeda Akash Fatima, Jiezhi Yang, Wanquan Chen, Taiguo Liu, Yuting Hu, Qing Li, Jingwei Guo, Min Zhang, Li Lei, Xin Li, Shengwen Tang, Peigao Luo

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Abstract

The wheat powdery mildew resistance gene Pm40, which is located on chromosomal arm 7BS, is effective against nearly all prevalent races of Blumeria graminis f. sp tritici (Bgt) in China and is carried by the common wheat germplasm PI 672538. A set of the F1, F2 and F2:3 populations from the cross of the resistant PI 672538 with the susceptible line L1034 were used to conduct genetic analysis of powdery mildew resistance and construct a highdensity linkage map of the Pm40 gene. We constructed a high-density linkage genetic map with a total length of 6.18 cM and average spacing between markers of 0.48 cM.Pm40 is flanked by Xwmc335 and BF291338 at genetic distances of 0.58 cM and 0.26 cM, respectively, in deletion bin C-7BS-1-0.27. Comparative genomic analysis based on EST-STS markers established a high level of collinearity of the Pm40 genomic region with a 1.09- Mbp genomic region on Brachypodium chromosome 3, a 1.16-Mbp genomic region on rice chromosome 8, and a 1.62-Mbp genomic region on sorghum chromosome 7. We further anchored the Pm40 target intervals to the wheat genome sequence. A putative linear index of 85 wheat contigs containing 97 genes on 7BS was constructed. In total, 9 genes could be considered as candidates for the resistances to powdery mildew in the target genomic regions, which encoded proteins that were involved in the plant defense and response to pathogen attack. These results will facilitate the development of new markers for mapbased cloning and marker-assisted selection of Pm40 in wheat breeding programs.

Original languageEnglish (US)
Article numbere0164815
JournalPloS one
Volume11
Issue number10
DOIs
StatePublished - Oct 2016

Bibliographical note

Funding Information:
We are grateful for the financial support of the National Natural Science Foundation of China (No. 31571661), the Specific Foundation of Agronomy (No. nyhyzx3-15, 201303016), the Bureau of Science and Technology of Fuling of Chongqing (FLKJ-2015ABB1048), and the Ministry of Science and Technology, China (2013CB127701). The funders had no role in the study design, data collection and analysis, the decision to publish, or the preparation of the manuscript.

Publisher Copyright:
© 2016 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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