Column selectivity in reversed-phase liquid chromatography: II. Effect of a change in conditions

N. S. Wilson; M. D. Nelson; J. W. Dolan; L. R. Snyder; P. W. Carr

doi:10.1016/S0021-9673(02)00660-X

Column selectivity in reversed-phase liquid chromatography: II. Effect of a change in conditions

N. S. Wilson, M. D. Nelson, J. W. Dolan, L. R. Snyder, P. W. Carr

Chemistry (Twin Cities)

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158 Scopus citations

Abstract

The isocratic retention of 67 widely-different solutes in reversed-phase liquid chromatography (RP-LC) has been investigated as a function of temperature and mobile phase composition (% B) for three different C₁₈ columns. Similar studies were also carried out in a gradient mode, where temperature, gradient time and solvent type were varied. These results show that changes in retention with these conditions are similar for each of these three columns. This suggests that relative column selectivity as defined by experiments for one set of experimental conditions will be approximately applicable for other conditions, with the exception of changes in mobile phase pH - which can affect values of the column parameter C (a measure of silanol ionization). Column selectivity as a function of pH was explored for several columns.

Original language	English (US)
Pages (from-to)	195-215
Number of pages	21
Journal	Journal of Chromatography A
Volume	961
Issue number	2
DOIs	https://doi.org/10.1016/S0021-9673(02)00660-X
State	Published - Jul 5 2002

Keywords

Column selectivity
Mobile phase composition
Retention prediction
Selectivity
Stationary phase, LC
Temperature effects

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title = "Column selectivity in reversed-phase liquid chromatography: II. Effect of a change in conditions",

abstract = "The isocratic retention of 67 widely-different solutes in reversed-phase liquid chromatography (RP-LC) has been investigated as a function of temperature and mobile phase composition (% B) for three different C18 columns. Similar studies were also carried out in a gradient mode, where temperature, gradient time and solvent type were varied. These results show that changes in retention with these conditions are similar for each of these three columns. This suggests that relative column selectivity as defined by experiments for one set of experimental conditions will be approximately applicable for other conditions, with the exception of changes in mobile phase pH - which can affect values of the column parameter C (a measure of silanol ionization). Column selectivity as a function of pH was explored for several columns.",

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T2 - II. Effect of a change in conditions

AU - Wilson, N. S.

AU - Nelson, M. D.

AU - Dolan, J. W.

AU - Snyder, L. R.

AU - Carr, P. W.

PY - 2002/7/5

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N2 - The isocratic retention of 67 widely-different solutes in reversed-phase liquid chromatography (RP-LC) has been investigated as a function of temperature and mobile phase composition (% B) for three different C18 columns. Similar studies were also carried out in a gradient mode, where temperature, gradient time and solvent type were varied. These results show that changes in retention with these conditions are similar for each of these three columns. This suggests that relative column selectivity as defined by experiments for one set of experimental conditions will be approximately applicable for other conditions, with the exception of changes in mobile phase pH - which can affect values of the column parameter C (a measure of silanol ionization). Column selectivity as a function of pH was explored for several columns.

AB - The isocratic retention of 67 widely-different solutes in reversed-phase liquid chromatography (RP-LC) has been investigated as a function of temperature and mobile phase composition (% B) for three different C18 columns. Similar studies were also carried out in a gradient mode, where temperature, gradient time and solvent type were varied. These results show that changes in retention with these conditions are similar for each of these three columns. This suggests that relative column selectivity as defined by experiments for one set of experimental conditions will be approximately applicable for other conditions, with the exception of changes in mobile phase pH - which can affect values of the column parameter C (a measure of silanol ionization). Column selectivity as a function of pH was explored for several columns.

KW - Column selectivity

KW - Mobile phase composition

KW - Retention prediction

KW - Selectivity

KW - Stationary phase, LC

KW - Temperature effects

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Column selectivity in reversed-phase liquid chromatography: II. Effect of a change in conditions

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Keywords

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