Community analysis and metabolic pathway of halophilic bacteria for phenol degradation in saline environment

Zhong zi Huang; Ping Wang; Hui Li; Kuang fei Lin; Zhi yan Lu; Xiao jue Guo; Yong di Liu

doi:10.1016/j.ibiod.2014.07.003

Community analysis and metabolic pathway of halophilic bacteria for phenol degradation in saline environment

Zhong zi Huang, Ping Wang, Hui Li, Kuang fei Lin, Zhi yan Lu, Xiao jue Guo, Yong di Liu

Bioproducts and Biosystems Engineering

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

A moderately halophilic bacterial enrichment was able to degrade 120mg/L of phenol in the presence of 1-2M of NaCl within 3d or 2.5-3M of NaCl within 6d. The optimal degradation was achieved at 1.5M of NaCl and 350mg/L of phenol. PCR-DGGE profile of the enrichment showed that the Acidobacterium sp. and Chloroflexus sp. dominated the community. The phenol-biodegradation pathways consisted of an initial oxidative attack by phenol hydroxylase, and subsequent ring fission by catechol 1,2-dioxygenase and catechol 2,3-dioxygenase. Nuclear magnetic resonance (NMR) spectroscopy profiles showed that ectoine and hydroxyectoine were the main compatible solutes to adjust the bacterial osmotic pressure. This study provides further information on the understanding of phenol-degradation over a wide range of salinity and remediation of phenol as a pollutant in the environment.

Original language	English (US)
Pages (from-to)	115-120
Number of pages	6
Journal	International Biodeterioration and Biodegradation
Volume	94
DOIs	https://doi.org/10.1016/j.ibiod.2014.07.003
State	Published - Oct 2014

Bibliographical note

Funding Information:
Supported jointly by National Natural Science Foundation of China ( 51378208 , 41273109 , 41003031 ), Specialized Research Fund for the Doctoral Program of Higher Education ( 20110074130002 ), Program for New Century Excellent Talents in University ( NCET-13-0797 ), Fok Ying Tung Education Foundation ( 141077 ), Shanghai Rising-Star Program ( 12QA1400800 ), Innovation Program of Shanghai Municipal Education Commission ( 14ZZ059 ), Fundamental Research Funds for the Central Universities ( 222201313008 ). We also would like to thank the anonymous referees for their helpful comments on this paper.

Keywords

Biodegradation
Compatible solutes
Metabolic pathway
Moderately halophilics
Phenol

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title = "Community analysis and metabolic pathway of halophilic bacteria for phenol degradation in saline environment",

abstract = "A moderately halophilic bacterial enrichment was able to degrade 120mg/L of phenol in the presence of 1-2M of NaCl within 3d or 2.5-3M of NaCl within 6d. The optimal degradation was achieved at 1.5M of NaCl and 350mg/L of phenol. PCR-DGGE profile of the enrichment showed that the Acidobacterium sp. and Chloroflexus sp. dominated the community. The phenol-biodegradation pathways consisted of an initial oxidative attack by phenol hydroxylase, and subsequent ring fission by catechol 1,2-dioxygenase and catechol 2,3-dioxygenase. Nuclear magnetic resonance (NMR) spectroscopy profiles showed that ectoine and hydroxyectoine were the main compatible solutes to adjust the bacterial osmotic pressure. This study provides further information on the understanding of phenol-degradation over a wide range of salinity and remediation of phenol as a pollutant in the environment.",

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author = "Huang, {Zhong zi} and Ping Wang and Hui Li and Lin, {Kuang fei} and Lu, {Zhi yan} and Guo, {Xiao jue} and Liu, {Yong di}",

note = "Funding Information: Supported jointly by National Natural Science Foundation of China ( 51378208 , 41273109 , 41003031 ), Specialized Research Fund for the Doctoral Program of Higher Education ( 20110074130002 ), Program for New Century Excellent Talents in University ( NCET-13-0797 ), Fok Ying Tung Education Foundation ( 141077 ), Shanghai Rising-Star Program ( 12QA1400800 ), Innovation Program of Shanghai Municipal Education Commission ( 14ZZ059 ), Fundamental Research Funds for the Central Universities ( 222201313008 ). We also would like to thank the anonymous referees for their helpful comments on this paper.",

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AU - Guo, Xiao jue

AU - Liu, Yong di

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PY - 2014/10

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N2 - A moderately halophilic bacterial enrichment was able to degrade 120mg/L of phenol in the presence of 1-2M of NaCl within 3d or 2.5-3M of NaCl within 6d. The optimal degradation was achieved at 1.5M of NaCl and 350mg/L of phenol. PCR-DGGE profile of the enrichment showed that the Acidobacterium sp. and Chloroflexus sp. dominated the community. The phenol-biodegradation pathways consisted of an initial oxidative attack by phenol hydroxylase, and subsequent ring fission by catechol 1,2-dioxygenase and catechol 2,3-dioxygenase. Nuclear magnetic resonance (NMR) spectroscopy profiles showed that ectoine and hydroxyectoine were the main compatible solutes to adjust the bacterial osmotic pressure. This study provides further information on the understanding of phenol-degradation over a wide range of salinity and remediation of phenol as a pollutant in the environment.

AB - A moderately halophilic bacterial enrichment was able to degrade 120mg/L of phenol in the presence of 1-2M of NaCl within 3d or 2.5-3M of NaCl within 6d. The optimal degradation was achieved at 1.5M of NaCl and 350mg/L of phenol. PCR-DGGE profile of the enrichment showed that the Acidobacterium sp. and Chloroflexus sp. dominated the community. The phenol-biodegradation pathways consisted of an initial oxidative attack by phenol hydroxylase, and subsequent ring fission by catechol 1,2-dioxygenase and catechol 2,3-dioxygenase. Nuclear magnetic resonance (NMR) spectroscopy profiles showed that ectoine and hydroxyectoine were the main compatible solutes to adjust the bacterial osmotic pressure. This study provides further information on the understanding of phenol-degradation over a wide range of salinity and remediation of phenol as a pollutant in the environment.

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Community analysis and metabolic pathway of halophilic bacteria for phenol degradation in saline environment

Abstract

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Keywords

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